Method of preparing a composition containing a high amount of ganglioside
AssigneeSnow Brand Milk Products Co., Ltd.
InventorsShuichi Yanahira, Kiyoshi Tatsumi
Life of this patent
Estimating the term…
Abstract
A method of preparing a composition containing a high amount of Ganglioside which comprises the steps of: (1) adding ethanol to a material containing Ganglioside so as to have a concentration of ethanol of 30 to 70 volume % and recovering the precipitated fraction containing Ganglioside; (2) adding ethanol to the recovered fraction containing Ganglioside so as to have a concentration of ethanol of 70 to 90 volume % and removing the precipitated proteins; and, (3) treating the fraction containing Ganglioside from which the proteins have been removed, with a membrane.
Claims6
A method of preparing a composition containing ganglioside which comprises the steps of:
- (1) extracting a material containing ganglioside with ethanol so that the concentration of ethanol is 30 to 70 volume %, and recovering a first precipitate,
- (2) reextracting the first precipitate with ethanol so that the concentration of ethanol is 70 to 90 volume %, and recovering resulting precipitated proteins, and
- (3) passing the liquid fraction from which the precipitated proteins had been removed resulting from step (2) through a membrane to yield a liquid fraction containing ganglioside.
Dependencies are inferred from the claim text.