Reconstituted platelet membrane vesicles
AssigneePRP, Inc.
InventorsFrancis C. Chao, Frank Huayueh Liang
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Abstract
A reconstituted platelet membrane vesicle preparation is provided. The preparation, directed at promoting hemostasis, may be prepared from proteins and lipids derived from either synthetic sources or from mammalian platelet membranes. The product may be used for transfusions or may be topically applied. Methods for forming the vesicles and products for storing and dispensing the vesicles also are provided.
Claims12
A method for preparing reconstituted platelet membrane vesicles for use in promoting hemostasis, comprising:
- combining a phospholipid preparation and a glycoprotein preparation;
- and
- mixing the phospholipid preparation and the glycoprotein preparation under conditions to form a sterile preparation of reconstituted membrane vesicles consisting essentially of a combination of phospholipid and glycoprotein, wherein the sterile preparation of reconstituted membrane vesicles is capable of promoting hemostasis.
A method for optimizing the glycoprotein:phospholipid ratio of reconstituted platelet membrane vesicles to yield membrane vesicles with maximal hemostatic efficacy, comprising:
- producing batches of reconstituted platelet membrane vesicles with different glycoprotein:phospholipid ratios;
- measuring the hemostatic properties of the batches;
- and
- selecting the batch having the glycoprotein:phospholipid ratio which yields vesicles with the highest measured hemostatic efficacy.
A method of optimizing the phospholipid composition of reconstituted platelet membrane vesicles to yield membrane vesicles with maximal incorporation of glycoproteins, comprising:
- producing batches of reconstituted platelet membrane vesicles with different phospholipid compositions;
- measuring the capability of the phospholipid composition to incorporate glycoprotein into the reconstituted membrane vesicles for each of the batches;
- and
- selecting the phospholipid composition which yields the reconstituted membrane vesicles with the highest incorporation of glycoproteins.
Dependencies are inferred from the claim text.