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AssigneeUniversity of Maryland, Baltimore
InventorsMordecai P. Blaustein, John M. Hamlyn, Douglas W. Harris, James H. Ludens, William Rodney Mathews, Jed F. Fisher, Frederic Mandel, Donald W. DuCharme
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Methods for diagnosing pre-hypertension, hypertension, congestive cardiomyopathy, renal failure, salt-sensitivity and adenomas and endocrine cell hyperplasias are disclosed. Also disclosed are methods for monitoring hypertension therapy, congestive cardiomyopathy therapy, renal failure therapy and adenoma and endocrine cell hyperplasia therapy. These methods involve using an antibody having binding specificity to ouabain to immunologically measure the level of human ouabain in body fluid or tissue of a subject. Additionally, methods for treating a hypertensive subject by inducing passive or active immunity to human ouabain in the subject are disclosed, along with an antibody having binding specificity for ouabain.
An antibody having binding specificity for human ouabain, wherein said antibody has a cross-reactivity for steroids present in human plasma on the order of about 1.0% or less cross-reactivity, wherein said antibody has a dissociation constant on the order of about 2.0 to 7.0 nM of plant ouabain.
A substantially purified human ouabain/which is capable of being detected in an immunoassay by an antibody having binding specificity for human ouabain, wherein said antibody has a cross-reactivity for steroids present in human plasma on the order of about 1.0% or less cross-reactivity.
Substantially pure human ouabain, wherein said human ouabain shows a protonated ion at m/z 495.1 in magnetic field/electric field linked scan mass spectra of the m/z 535 parent ions, which protonated ion is absent in the spectra of plant ouabain.
Substantially pure human ouabain obtained from human body fluid or human tissue sample.
Substantially pure human ouabain, wherein said human ouabain is obtainable by the process comprising the steps of:
Substantially pure human ouabain, wherein said human ouabain is obtainable by the process comprising the steps of:
A process for obtaining human ouabain comprising the steps of:
A process for obtaining human ouabain comprising the steps of:
Dependencies are inferred from the claim text.