Modified hemoglobin-like compounds
AssigneeSomatogen, Inc.
InventorsDavid C. Anderson, Antony J. Mathews, Stephen P. Trimble, Spencer Anthony-Cahill
Life of this patent
Estimating the term…
Abstract
The present invention relates to modified hemoglobin-like compounds. The novel compounds include a globin-like polypeptide containing at least two di-alpha domains and multimeric hemoglobin-like proteins having a core hemoglobin-like moiety directly attached to at least two other hemoglobin-like moieties. The invention also relates to nucleic acid molecules encoding the novel polypeptides. Methods of making the multimeric hemoglobin-like proteins are also provided, as well as compositions containing the proteins.
Claims23
A purified globin-like polypeptide comprising two di-alpha domains.
A multimeric hemoglobin-like protein comprising a core hemoglobin-like moiety to which each of at least two other hemoglobin-like moieties are directly attached to the core hemoglobin-like moiety.
A method for making a multimeric hemoglobin-like protein, comprising:
- (a) obtaining a first hemoglobin-like moiety having an amino acid capable of attaching to one end of a heterobifunctional linker to form a core hemoglobin-like moiety;
- (b) obtaining at least two other hemoglobin-like moieties having an amino acid capable of attaching to the other end of the heterobifunctional linker;
- (c) contacting the heterobifunctional linker to the core hemoglobin-like moiety to form a linked moiety;
- and
- (d) contacting the other hemoglobin-like moieties to the linked moiety to form the multimeric hemoglobin-like protein.
A composition comprising a multimer hemoglobin-like protein having a core hemoglobin-like moiety to which each of at least two other hemoglobin-like moieties are directly attached to the core hemoglobin-like moiety.
Dependencies are inferred from the claim text.