Loading patent…
AssigneesThe Salk Institute for Biological Studies, University of Reading
InventorsEllen Potter, Dominic P. Behan, Wolfgang H. Fischer, Elizabeth A. Linton, Philip J. Lowry, Wylie W. Vale, Jr.
Estimating the term…
A Corticotropin Releasing Factor-binding protein (CRF-BP) is isolated and purified sufficient to provide AA sequence data from which the DNA is obtained, which is then used to produce the protein recombinantly. The CRF-BP is useful for modulating the biological activity of CRF, such as reducing the high ACTH levels in mammals caused by excess CRF. The CRF-BP or fragments thereof and/or antibodies to the proteins may be employed in diagnostic assays to determine the levels of CRF, CRF-BP and the ratio of CRF/CRF-BP in a vascular fluid sample. The DNA or subsequence thereof can be used as probes for genetic material in certain assays. The anti-CRF-BP antibodies are also useful to purify the CRF-BP protein and to modulate the biological effect of CRF-BPs proteins.
An at least about 98% pure protein which has amino acid SEQ ID NO:1, or which is a biologically active fragment thereof which is shortened by elimination of a sequence beginning at the C-terminus and/or the N-terminus, which fragment binds to hCRF and modulates the biological activity thereof.
Substantially pure recombinant human CRF binding protein recovered from recombinant host cells transformed with a replicable recombinant DNA expression vector which vector includes an isolated and purified DNA fragment encoding a CRF binding protein having amino acid SEQ ID NO:1 and is capable of expressing said DNA fragment.
Dependencies are inferred from the claim text.