Anionic saccharides for extraction of anti-angiogenic protein from cartilage
AssigneeBioCell Technology, LLC
InventorPaul B. Weisz
Life of this patent
Estimating the term…
Abstract
Anti-angiogenic protein composition can be prepared simply and efficiently by contacting cartilage particles containing the protein together with an electrolyte solution and an oligosaccharide bearing a minimum number of anionic substituents per sugar unit, to extract the anti-angiogenic protein from the cartilage particles and to form a complex of the oligosaccharide and the protein. The resultant anti-angiogenic complex can be used for therapeutic treatment of various angiogenic and related diseases.
Claims20
A method of extracting an anti-angiogenic protein from cartilage comprising the steps of:
- contacting particles of cartilage with (a) a saccharide having a suitable degree of intramolecular density of anionic substituents and (b) an aqueous medium containing between 0.2 and 4.0 molar concentration of inorganic cations for a time sufficient for anti-angiogenic protein transfer from the cartilage to the saccharide to form a composition, and
- separating said composition containing extracted anti-angiogenic protein.
A method of extracting an anti-angiogenic protein from cartilage comprising the steps of:
- contacting particles of cartilage with a composition containing a saccharide having a suitable degree of intramolecular density of anionic substituents and an aqueous medium containing from about 0.2 to 4.0 molar concentration of inorganic cations,
- allowing sufficient time for the anti-angiogenic protein from cartilage particles to complex with said saccharide,
- separating saccharide complexed with protein, then
- removing said anti-angiogenic protein from the saccharide/protein complex by contacting the solution containing said complex with an inorganic salt at sufficiently high salt concentration to release the protein from the complex.
Dependencies are inferred from the claim text.