Method for assaying photosensitizing drug in whole blood
OwnerAvigdor M. Ronninventor
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Abstract
A concentration of a photosensitizing drug in a patient, for PhotoDynamic Therapy treatment of the patient by a treatment light at a treatment light wavelenght, is determined by the steps of drawing a blood sample from the patient, spreading the blood sample between two optically transmissive plates to form a spread sample, irradiating the spread sample with an interrogation light at an interrogation wavelength range of an absorption band bluer than the treatment light wavelength, measuring a magnitude of an emission signal at the treatment light wavelength, the emission signal caused by the irradiation of the spread sample, and comparing the measured magnitude to a magnitude/concentration calibration curve to determine the concentration of the photosensitizing drug.
Claims18
A method for determining a concentration of a photosensitizing drug in a patient, for PhotoDynamic Therapy treatment of said patient by a treatment light at a treatment light wavelength, said method comprising the steps of:
- drawing a whole blood sample from said patient;
- spreading said whole blood sample between two optically transmitting plates to form a spread sample containing some amount of a photosensitizing drug including none;
- irradiating said spread sample with an interrogation light in an interrogation wavelength range of an absorption band, of said photosensitizing drug, bluer than said treatment light wavelength;
- measuring a magnitude of an emission signal at said treatment light wavelength, said emission signal caused by said irradiation of said spread sample;
- and
- comparing said measured magnitude to a magnitude/concentration calibration curve to determine the concentration of said photosensitizing drug.
A method for determining a concentration of a photosensitizing drug in a patient, for PhotoDynamic Therapy treatment of said patient by a treatment light at a treatment light wavelength, said method comprising the steps of:
- drawing a whole blood sample from said patient;
- spreading said whole blood sample between two optically transmitting plates to produce a spread sample containing some amount of a photosensitizing drug including none;
- drying said spread sample to produce a dried spread sample;
- irradiating said dried spread sample with an interrogation light in an interrogation wavelength range of an absorption band, of the photosensitizing drug, bluer than said treatment light wavelength;
- measuring a magnitude of a measurable emission signal at said treatment light wavelength, said measurable emission signal caused by said irradiation of said dried spread sample;
- and
- comparing said measured magnitude to a magnitude/concentration calibration curve to determine the concentration of said photosensitizing drug.
A method for determining a concentration of a photosensitizing drug in a patient, for PhotoDynamic Therapy treatment of said patient by a treatment light at a treatment light wavelength, said method comprising the steps of:
- drawing a calibrated whole blood sample from said patient;
- spreading said calibrated whole blood sample between two optically transmitting plates to form a spread sample containing some amount of a photosensitizing drug including none;
- irradiating said spread sample with an interrogation light in an interrogation wavelength range of an absorption band, of said photosensitizing drug, bluer than said treatment light wavelength;
- measuring a magnitude of an emission signal at said treatment light wavelength, said emission signal caused by said irradiation of said spread sample;
- and
- comparing said measured magnitude to a magnitude/concentration calibration curve to determine the concentration of said photosensitizing drug.
Dependencies are inferred from the claim text.