To permit detection of trace unstable reaction products to clarify the reaction process of oxygenation, this invenion provides a method of introducing positron nuclide .sup.15 O as an oxygen additive into the reaction system.
Drawings
Claims5
In a method of detecting reaction products in an enzymatic oxygenation reaction system, the improvement which comprises introducing positron nuclide .sup.15 O as an additive oxygen to the enzymatic reaction system and detecting radioactive products produced during the oxygenation reaction by use of radio-UV-LC-MS (liquid chromatography mass spectrometry with radio absorption detection).
13 Sep 2016Estimated · howhide20 years from earliest known nonprovisional domestic filing. Maintenance-fee lapses, terminal disclaimers and statutory extensions have not been checked. The continuity chain may be incomplete; this is not an enforceability determination. Patent term adjustment is unavailable; no adjustment was added.
Measuring or testing processes involving enzymes, nucleic acids or microorganisms
Life of this patent
Term ended 13 Sep 2016 (estimated)
Priority13 Sep 1996
Granted1 Dec 1998
Term ended13 Sep 2016
Priority13 Sep 1996
Granted1 Dec 1998
Term ended13 Sep 2016
Application filedUS 08/713,479
GrantedUS 5,843,784 A
Term endedEstimated
USPTO file history isn’t available for this patent, so only indexed dates are shown.
Description1,293 words
Summary550 words
#The present invention relates to a radio-detection method of oxygenation. More particularly, the present invention relates to a novel radio-detection method which permits easier detection of trace metabolites even in a crude substance system, and allows clarification of mechanism of the oxygenation, oxidation and biochemical applications.
#Oxygen is an essential molecule for life. Recently, the general attention is attracted by many biochemical changes brought about by the result of oxidation stress as one of senescence mechanisms, and participation of oxygen in biomolecules is becoming an important problem. When considering metabolism as viewed from the point of view of oxygen, there have often been referred to the facts that cellular respiration produces energy (ATP), that it is important for biosynthesis of many physiologically active substances such as steroids, prostaglandin, catecholamine and serotonin by the action of a enzymatic oxygenation, and radicals produced from oxygen. There as however almost unavailable a report viewing these metabolites from oxygen molecule itself. This is attributable to the absence of a radioisotope easy to use for oxygen, as described later.
#While enzyme plays an important role in biochemical reactions, on the other hand, it is not so easy to analyze its process of reaction. For example, the oxygenation is known as a reaction caused by enzyme, and the process of reaction is attracting researchers' interest as a reaction using oxygen in cells and bio-tissues. Actually, however, almost no means of its analysis has as yet been practicable. This is due to the fact that detection of trace unstable metabolites occurring in the course of reaction is very difficult.
Brief description of the drawings3 figures
#FIG. 1 shows a configurational diagram illustrating an experimental system;
#FIG. 2 shows a graph illustrating the relationship between the reaction time in an example and the detection radioactivity and
#The present invention makes it possible to, in oxygenation involving various substances, detect trace unstable metabolites or reaction intermediates which has conventionally been difficult to detect. It is thus possible to apply the present invention to clarification of reaction mechanism, studies on functions displayed in biological cells and tissues, and to various medical and biochemical uses as well as to chemical synthesis.
#While .sup.15 O has a relatively short physical half-life of 2.07 minutes, the specific radioactivity is high (theoretically 3.36.times.10.sup.21 Bq/mol), so that it is very advantageous for search to trace unstable metabolites.
#The label by .sup.15 O is positron nuclide: for example, the one used for positron emission tomography is applicable, which may be introduced during oxygenation reaction.