Micropropagation of rose plants
AssigneeNovaFlora Inc.
InventorsMichael Dobres, Gail R. Williams Lokaj
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AssigneeNovaFlora Inc.
InventorsMichael Dobres, Gail R. Williams Lokaj
Estimating the term…
The present invention is directed to, inter alia, a method of micropropagation of a rose plant comprising culturing a stem bearing a node in a first enclosed vessel containing a first culture medium comprising about 0.5 to about 3.0 mg/l of a cytokine, 0 to about 1.0 mg/l of an auxin, and 0 to about 1.0 mg/l of a gibberellin until at least one shoot emerges from the node; excising the shoot from the stem and propagating the shoot in a second enclosed vessel containing a second culture medium comprising about 0.5 to about 3.0 mg/l of a cytokine, 0 to about 1.0 mg/l of an auxin, and 0 to about 1.0 mg/l of a gibberellin to produce a flowering rose plant capable of being transferred to soil. Among other things, the present invention is also directed to rose plants and culture media for the micropropagation of roses.
A method of tissue culture micropropagation of a rose plant comprising culturing a stem bearing a node in a first enclosed vessel containing a first culture medium comprising about 0.5 to about 3.0 mg/l of a cytokine, 0 to about 1.0 mg/l of an auxin, and 0 to about 1.0 mg/l of a gibberellin until at least one shoot is produced from said node;
The method of 7 wherein said culture media comprises about 0.5 to about 3.0 mg/l of benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
The method of 7 wherein said culture media comprises about 1.0 mg/l benzyladenine.
The method of 7 wherein said culture media comprises about 2.0 mg/l benzyladenine.
The method of 7 wherein said culture media comprises about 3.0 mg/l benzyladenine.
A method of tissue culture micropropagation of a rose plant comprising culturing a stem bearing a node in an enclosed vessel containing culture medium comprising about 0.5 to about 3.0 mg/l benzyladenine, 0 to about 1.0 mg/l indole acetic acid, and 0 to about 1.0 mg/l gibberellic acid, wherein said stem is cultured until at least one shoot is produced from said node, said shoot produced from said node capable of producing a rose plant bearing a flower, or until a flowering rose plant is produced in said enclosed vessel.
A method of tissue culture micropropagation of a rose plant comprising culturing a shoot in an enclosed vessel containing culture medium comprising about 0.5 to about 3.0 mg/l benzyladenine, 0 to about 1.0 mg/l indole acetic acid, and 0 to about 1.0 mg/l gibberellic acid, wherein said shoot is cultured until a flowering rose plant is produced in said enclosed vessel.
A culture medium for tissue culture micropropagation of a rose plant comprising about 0.5 to about 3.0 mg/l benzyladenine, 0 to about 1.0 mg/l indole acetic acid, and 0 to about 1.0 mg/l gibberellic acid.
The culture medium of 35 comprising about 0.5 to about 3.0 mg/l of benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
The culture medium of 35 comprising about 1.0 mg/l benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
The culture medium of 35 comprising about 2.0 mg/l benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
The culture medium of 35 comprising about 3.0 mg/l benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
A rose culture comprising a rose stem bearing a node contained in an enclosed vessel containing culture medium comprising about 0.5 to about 3.0 mg/l benzyladenine, 0 to about 1.0 mg/l indole acetic acid, and 0 to about 1.0 mg/l gibberellic acid, wherein said rose culture is capable of generating a flowering rose plant in said enclosed vessel.
The rose culture of 42 comprising about 0.5 to about 3.0 mg/l of benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
The rose culture of 42 comprising about 1.0 mg/l benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
The rose culture of 42 comprising about 2.0 mg/l benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
The rose culture of 42 comprising about 3.0 mg/l benzyladenine, about 0.3 mg/l of indole acetic acid, and about 0.1 mg/l gibberellic acid.
A rose explant selected from the group consisting of a rose stem bearing a node, a rose stem bearing a node and at least one shoot, and a rose shoot, said rose explant contained in an enclosed vessel comprising a culture medium comprising about 0.5 to about 3.0 mg/l benzyladenine, 0 to about 1.0 mg/l indole acetic acid, and 0 to about 1.0 mg/l gibberellic acid, wherein said rose explant is capable of producing a flowering rose plant in said enclosed vessel.
A rooting medium for the rooting of rose plants comprising MS medium and about 1 to about 5 mg/l glycine, about 50 to about 150 mg/l myo-inositol, about 0.1 to about 1.0 mg/l nicotinic acid, about 0.1 to about 1.0 mg/l pyridoxine.HCl, about 0.01 to about 0.2 mg/l thiamine.HCl, about 10 to about 50 g/l sucrose, and optionally containing about 1 to about 5 g/l gelling agent, about 0.05 to about 1 mg/l indole butyric acid.
Dependencies are inferred from the claim text.