Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions
AssigneeHouston Advanced Research Center
InventorKenneth L. Beattie
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Abstract
An improved microfabricated apparatus for conducting a multiplicity of individual and simultaneous binding reactions is described. The apparatus comprises a substrate on which are located discrete and isolated sites for binding reactions. The apparatus is characterized by discrete and isolated regions that extend through said substrate and terminate on a second surface thereof such that when a test sample is allowed to the substrate, it is capable of penetrating through each such region during the course of said binding reaction. The apparatus is especially useful for sequencing by hybridization of DNA molecules.
Claims38
A device for binding a target molecule, comprising:
- a substrate having oppositely facing first and second major surfaces;
- a multiplicity of discrete channels extending through said substrate from said first major surface to said second major surface;
- a first binding reagent immobilized on the walls of a first group of said channels, and
- a second binding reagent immobilized on the walls of a second group of said channels.
A device for binding a target molecule, comprising:
- a substrate having oppositely facing first and second major surfaces;
- a multiplicity of discrete channels extending through said substrate from said first major surface to said second major surface;
- a first binding reagent immobilized on the walls of a first group of said channels, and
- a second binding reagent immobilized on the walls of a second group of said channels,
- further comprising a rigid support, wherein said rigid support is integral to said substrate, or is bonded to said substrate.
Dependencies are inferred from the claim text.