Enzyme based bioremediation
AssigneeThe Commonwealth of Australia Commonwealth Scientific and Industrial Research Organization
InventorsRobyn Joyce Russell, Richard David Newcomb, Geoffrey Charles de Quetteville Robin, Thomas Mark Boyce, Peter Malcolm Campbell, Anthony Gerard Parker, John Graham Oakeshott, Kerrie-Ann Smyth
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Abstract
The present invention provides an E3 esterase from an organophosphate resistant strain of L. cuprina. The invention further provides DNA sequences encoding such E3 esterases and cells transformed with the DNA sequences. In addition, the invention relates to the use of the enzyme and transformed cells as bioremediation agents.
Claims17
An isolated and purified organophosphate (OP)-resistant E3 esterase having the amino acid sequence shown in SEQ ID NO:
- 4 or an ortholog thereof, wherein said OP-resistant E3 esterase has a substitution of the amino acid corresponding to the glycine residue at position 137 of the OP-sensitive E3 esterase from Lucilia cuprina having the amino acid sequence of SEQ ID NO:
- 3.
An isolated DNA molecule comprising the nucleotide sequence shown in SEQ ID NO:
- 2 or an ortholog thereof, which encodes an OP-resistant E3 esterase that hydrolyses organophosphate pesticide residues, carbamate pesticide residues, or organophosphate and carbamate pesticide residues, wherein said DNA molecule encodes an OP-resistant E3 esterase having a substitution of the amino acid corresponding to the glycine residue at position 137 of the OP-sensitive E3 esterase from L. cuprina having the amino acid sequence of SEQ ID NO:
- 3.
A method of determining the susceptibility of a strain of L. cuprina to organophosphates comprising:
- isolating and cloning from the strain the gene encoding E3 esterase;
- and
- comparing the sequence of the E3 encoding gene with gene Lc.alpha.E7 encoding OP-sensitive E3 esterase.
An isolated and purified OP-resistant E3 esterase which has a substitution of the amino acid corresponding to the glycine residue at position 137 of the OP-sensitive E3 esterase from L. cuprina having the amino acid sequence of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.