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US 5,843,753 AGranted
Metalloprotease having increased activity
AssigneesNovo Nordisk A/S, Novo Nordisk Biotech, Inc.
InventorsJeffrey R. Shuster, Mark Madden, Donna L. Moyer, Claus Fuglsang, Sven Branner
Life of this patent
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Abstract
The present invention relates to a novel metalloprotease obtainable from a fungus having increased proteolytic activity. Additionally, the invention related to isolated nucleic acid fragments encoding said metalloprotease as well as vectors, DNA constructs, and recombinant host cells comprising said nucleic acid fragments.
Claims8
A substantially pure metalloprotease obtained from Fusarium having the following characteristics:
- (a) a molecular weight from about 45,000 daltons to about 50,000 daltons as determined by SDS polyacrylamide gel electrophoresis;
- (b) functions optimally at a pH between about 8.0 and 11.0;
- (c) is at least about 10 times more effective than a metalloprotease obtained from Bacillus stearothermophilus in converting a proenzyme to an active trypsin-like protease obtained from a strain of F. oxysporum deposited at the Deutsche Sammlung von Mikroorganismen, Gottingen, Germany under the number DSM 2672 at a pH between about 6.0 and 7.5 at about 25.degree.-30.degree. C. for about 30-60 minutes;
- and (d) is less effective than a metalloprotease obtained from Bacillus stearothermophilus in cleaving the primary amino groups from casein.
1 more element
Dependencies are inferred from the claim text.