Sorbitol kinase, process for producing the same, and substantially pure microorganism
AssigneeAsahi Kasei Kogyo Kabushiki Kaisha
InventorsShinji Koga, Mamoru Takahashi, Kouji Suzuki
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Abstract
A sorbitol kinase having at least the following physicochemical properties: (1) Enzymatic action: utilizing mainly ATP, but not substantially phosphoenolpyruvate, as the phosphate donor catalyzing a reaction of generating sorbitol-6-phosphate and ADP from sorbitol and ATP of the formula, sorbitol+ATP.fwdarw.sorbitol-6-phosphate+ADP (2) Substrate specificity: for sorbitol (3) N-terminal amino acid sequence: containing ##STR1## (4) Heat stability stable at least up to 60.degree. C. when a 100 mM Tris-HCl buffer (pH 8.5, containing 0.5 U/ml of sorbitol kinase) is heated for 15 minutes at various temperatures and the residual acitvity is measured.
Claims6
An isolated and purified sorbitol kinase having at least the following physicochemical properties:
- (a) utilizing mainly ATP, but not substantially phosphoenolpyruvate, as the phosphate donor catalyzing a reaction of generating sorbitol-6-phosphate and ADP from sorbitol and ATP of the formula,
- sorbitol+ATP.fwdarw.sorbitol-6-phosphate+ADP
- (b) substrate specificity for sorbitol
- (c) N-terminal amino acid sequence containing ##STR5## (d) heat stable at least up to 60.degree. C. when a 100 mM Tris-HCl buffer (pH 8.5, containing 0.5 U/ml of sorbitol kinase) is heated for 15 minutes at various temperatures and the residual acitvity is measured.
An isolated and purified sorbitol kinase having the amino acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.