Trehalose phosphorylase its preparation and uses
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsTetsuya Nakada, Michio Kubota, Hiroto Chaen, Toshio Miyake
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Abstract
A thermostable trehalose phosphorylase which is obtainable from microorganisms of the genus Thermoanaerobium and which hydrolyzes trehalose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid. The trehalose phosphorylase can be also prepared by recombinant DNA technology. When the enzyme is allowed to contact with .beta.-D-glucose-1-phosphoric acid as a saccharide donor in the presence of other saccharides, glucosyl-transferred saccharides including glucosyl-D-galactoside, which are conventionally known but scarcely obtainable, can be produced on an industrial-scale and in a relatively-low cost.
Claims10
A purified trehalose phosphorylase which is obtainable from a microorganism of the genus Thermoanaerobium and which hydrolyzes trehalose in the presence of a compound selected from the group consisting of an inorganic phosphoric acid, a salt thereof, and mixture thereof to form D-glucose and a compound selected from the group consisting of .beta.-D-glucose-1-phosphoric acid, a salt thereof, and a mixture thereof, said trehalose phosphorylase having a thermal stability at about 60.degree. C. when incubated at pH 7.0 for one hour.
Dependencies are inferred from the claim text.