Back to results
US 5,843,741 AGranted
Method for altering the differentiation of anchorage dependent cells on an electrically conducting polymer
AssigneesMassachusetts Insitute of Technology, Children's Medical Center Corporation
InventorsJoyce Y. Wong, Donald E. Ingber, Robert S. Langer
Life of this patent
Estimating the term…
Abstract
Described is a method and cell culture system for altering the proliferation, differentiation, or function of anchorage dependent cells which includes associating the cells with a surface formed of an electrically conducting polymer and applying an effective amount of a voltage to change the oxidation state of the polymer without damaging the cells.
Claims26
A method for altering the differentiation of anchorage dependent cells comprising
- binding cell surface receptor-specific attachment molecules of defined types and in a defined density to a surface consisting of an electrically conducting polymer,
- attaching anchorage-dependent cells to the cell surface receptor-specific attachment molecules on the electrically conducting polymer surface, and
- applying a voltage in an effective amount to change the oxidation state of the electrically conducting polymer thereby to alter the differentiation as a function of the type and density of the attachment molecules and applied voltage,
- wherein the anchorage dependent cells are selected from the group consisting of animal cells and plant cells.
1 more element
A device or structure for altering the differentiation of anchorage dependent cells comprising:
- an electrically conductive polymer surface;
- cell surface receptor-specific attachment molecules of defined types bound to the electrically conductive polymer surface in a defined density;
- and
- a means to apply a voltage across the electrically conductive polymer surface, wherein the means is electrically connected with the polymer,
- wherein the effect of the voltage on the differentiation of the cells attached to the polymer surface is altered as a function of the type and density of the attachment molecules.
2 more elements
Dependencies are inferred from the claim text.