DNA encoding 2-acyltransferases
AssigneeNickerson Biocem Limited
InventorsAntoni Ryszard Slabas, Adrian Paul Brown
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Abstract
Plants, particularly transgenic plants, may be produced having a 2-acyltransferase enzyme or other insoluble acyltransferase enzyme with an altered substrate specificity compared to the native enzyme. For example, oil seed rape (Brassica napus) may contain a 2-acyltransferase transgene derived from Limnanthes douglassi in order to increase the erucic acid content of the oil. The cDNA sequence of maize (Zea mays) 2-acyltansferase is disclosed and is useful for cloning acyltransferase genes and/or cDNAs from other organisms, including L. douglassi.
Claims13
A recombinant or isolated nucleic acid molecule comprising a polynucleotide sequence selected from the group consisting of
- (a) a nucleotide sequence encoding the polypeptide having the amino acid sequence in FIGS. 1A-1C (SEQ ID NO:
- 2) from about amino acid 1 to about amino acid 374 in SEQ ID NO:
- 2
- (b) a nucleotide sequence complementary to a nucleotide sequence encoding the polypeptide having the amino acid sequence in FIGS. 1A-1C (SEQ ID NO:
- 2) from about amino acid 1 to about amino acid 374 in SEQ ID NO:
- 2;
- (c) a nucleotide sequence which hybridizes to the nucleotide sequence in FIGS. 1A-1C (SEQ ID NO:
- 1) under stringent conditions;
- and
- (d) a nucleotide sequence which hybridizes to the nucleotide sequence that is complementary to the nucleotide sequence in FIGS. 1A-1C (SEQ ID NO:
- 1) under stringent conditions.
Dependencies are inferred from the claim text.