Aklavinone C-11 hydroxylase, gene coding for same, expression vector therefor, and process for preparing hybrid antibiotics by using said vector
AssigneesKorea Institute of Science and Technology, Il Dong Pharmaceutical Co., Ltd.
InventorsJung-Joon Lee, Young-Ho Kim, Soon-Kwang Hong, Young-Soo Hong, Cheol-Kyu Hwang, Hang-Sub Kim
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Abstract
Aklavinone C-11 hydroxylase separated from Streptomyces sp.; a gene encoding same; an expression vector comprising said gene; a microorganism transformed with said vector; and a process for preparing novel hybrid anthracyclines by using said vector.
Claims10
An isolated aklavinone C-11 hydroxylase having SEQ ID NO :3.
An isolated polynucleotide sequence encoding aklavinone C-11hydroxylase having SEQ ID NO:4.
The Streptomyces galilaeous strain ATCC 31133 transformed with plasmid pMC213.
A process for the preparation of an 11-hydroxy aclacinomycin comprising:
- transforming Streptomyces galilaeous ATCC 31133 with plasmid pMC213 ;
- culturing the Streptomyces galilaeous strain ATCC 31133 transformed with
- the plasmid pMC213 for a time and under suitable conditions to produce said aclacinomycin and recovering said aclacinomycin.
An aclacinomycin compound X of the formula. ##STR3##
An aclacinomycin compound X of the formula. ##STR4##
Dependencies are inferred from the claim text.