Redirection of cellular immunity by receptor chimeras
AssigneeThe General Hospital Corporation
InventorsBrian Seed, Charles Romeo, Waldemar Kolanus
Life of this patent
Estimating the term…
Abstract
Disclosed is a method of directing a cellular response in a mammal by expressing in a cell of the mammal a chimeric receptor which causes the cells to specifically recognize and destroy an infective agent, a cell infected with an infective agent, a tumor or cancerous cell, or an autoimmune-generated cell. Also disclosed are cells which express the chimeric receptors and DNA encoding the chimeric receptors.
Claims55
DNA encoding a membrane-bound, proteinaceous chimeric receptor comprising (a) an extracellular portion which specifically recognizes and binds a target cell or a target infective agent and (b) an intracellular portion of a T cell receptor CD3, .zeta., or .eta. polypeptide, a B cell receptor polypeptide, or an Fc receptor polypeptide.
DNA encoding a membrane-bound, proteinaceous chimeric receptor comprising (a) an extracellular portion which specifically recognizes and binds a target cell or a target infective agent and (b) a transmembrane portion of a T cell receptor CD3, .zeta., or .eta. polypeptide, a B cell receptor polypeptide, or an Fc receptor polypeptide.
DNA having the sequence shown in SEQ ID NO:3.
A membrane-bound, proteinaceous chimeric receptor, comprising (a) an extracellular portion which specifically recognizes and binds a target cell or a target infective agent and (b) an intracellular portion of a T cell receptor CD3, .zeta., or .eta. polypeptide, a B cell receptor polypeptide, or an Fc receptor polypeptide.
A membrane-bound, proteinaceous chimeric receptor, comprising (a) an extracellular portion which specifically recognizes and binds a target cell or a target infective agent and (b) a transmembrane portion of a T cell receptor CD3, .zeta., or .eta. polypeptide, a B cell receptor polypeptide, or an Fc receptor polypeptide.
A proteinaceous membrane-bound chimeric receptor, said receptor comprising (a) an extracellular CD4 portion which specifically recognizes and binds a target cell or a target infective agent, and (b) an intracellular portion derived from a T cell receptor, a B cell receptor, or an Fc receptor which is capable of signals said cell to destroy a receptor-bound target cell or receptor-bound infective agent.
A proteinaceous membrane-bound chimeric receptor, said receptor comprising (a) an extracellular CD4 portion which specifically recognizes and binds a target cell or a target infective agent, and (b) a transmembrane portion derived from a T cell receptor, a B cell receptor, or an Fc receptor which is capable of signals said cell to destroy a receptor-bound target cell or receptor-bound infective agent.
A polypeptide selected from the group consisting of T cell receptor .zeta. amino acids 421-532, 423-455, 438-455, 461-494, 494-528, or 400-420 of SEQ ID NO:
- 6.
A cell which expresses a proteinaceous membrane-bound chimeric receptor, said receptor comprising (a) an extracellular portion which specifically recognizes and binds a target cell or a target infective agent, and (b) an intracellular portion derived from a T cell receptor CD3, .zeta., or .eta. polypeptide, a B cell receptor, or an Fc receptor which signals said cell to destroy a receptor-bound target cell or receptor-bound target infective agent.
A cell which expresses a proteinaceous membrane-bound chimeric receptor, said receptor comprising (a) an extracellular portion which is capable of specifically recognizing and binding a target cell or a target infective agent, and (b) a transmembrane portion derived from a T cell receptor CD3, .zeta., or .eta. polypeptide, a B cell receptor, or an Fc receptor which is capable of signalling said cell to destroy a receptor-bound target cell or receptor-bound target infective agent.
A cell which expresses a proteinaceous membrane-bound chimeric receptor, said receptor comprising (a) an extracellular CD4 portion which specifically recognizes and binds a target cell or a target infective agent, and (b) an intracellular portion derived from a T cell receptor, a B cell receptor, or an Fc receptor which is capable of signals said cell to destroy a receptor-bound target cell or receptor-bound infective agent.
A cell which expresses a proteinaceous membrane-bound chimeric receptor, said receptor comprising (a) an extracellular CD4 portion which is capable of specifically recognizing and binding a target cell or a target infective agent, and (b) a transmembrane portion derived from a T cell receptor, a B cell receptor, or an Fc receptor which is capable of signalling said cell to destroy a receptor-bound target cell or receptor-bound infective agent.
A cell which expresses a proteinaceous membrane-bound chimeric receptor comprising (a) an extracellular portion which specifically recognizes and binds a target cell or a target infective agent and (b) an intracellular portion of a T cell receptor CD3, .zeta., or .eta. polypeptide, a B cell receptor polypeptide, or an Fc receptor polypeptide.
A cell which expresses a proteinaceous membrane-bound chimeric receptor comprising (a) an extracellular portion which specifically recognizes and binds a target cell or a target infective agent and (b) a transmembrane portion of a T cell receptor CD3, .zeta., or .eta. polypeptide, a B cell receptor polypeptide, or an Fc receptor polypeptide.
Dependencies are inferred from the claim text.