Recombinant production of novel polyketides
AssigneesLeland Stanford Junior University, The John Innes Institute
InventorsChaitan Khosla, David A. Hopwood, Susanne Ebert-Khosla, Robert McDaniel, Hong Fu
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Abstract
Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.
Claims21
A host cell containing nucleic acid molecules, wherein said molecules comprise a replacement polyketide synthase (PKS) gene cluster functional in catalyzing the synthesis of a polyketide, said gene cluster comprising a first open reading frame (ORF) encoding a PKS ketosynthase and a PKS acyltransferase (KS/AT), a second ORF encoding a PKS chain length determining factor (CLF), a third ORF encoding a PKS acyl carrier protein (ACP) and, one or more control sequences operatively linked to said PKS gene cluster, whereby the ORFs can be transcribed and translated to produce a functional PKS,
- wherein at least one of the ORFs or control sequences is heterologous to the cell and
- wherein the native PKS gene cluster has been deleted from the host cell using recombinant DNA techniques.
Dependencies are inferred from the claim text.