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AssigneeKirin Beer Kabushiki Kaisha
InventorsAruto Yoshida, Makoto Takeuchi
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An amino acid sequence that can specifically introduce a mucin type sugar chain into a protein or peptide chain and a technique of introducing a mucin type sugar chain into protein or peptide by utilizing such a sequence are disclosed. GalNAc moiety of UDP-GalNAc (where UDP represents uridine 5'-diphosphate and GalNAc represents N-acetylgalactosamine) is introduced into the amino acid X(O) in the presence of UDP-GalNAc: polypeptide .alpha.1, O-GalNAc transferase (O-GalNAc T): X(-1)-X(0)-X(1)-X(2)-X(3) (I) where X(-1) and X(2) represent independently any amino acid, X(O) represents T or S and X(1) and X(3) represent independently any amino acid except that at least one of X(1) and X(3) represents P.
A method for producing a modified protein or peptide having a mucin-type sugar chain, comprising culturing eukaryotic cells transformed with a chimeric DNA molecule encoding said modified protein or peptide, and collecting said modified protein or peptide produced by said eukaryotic cells,
A chimeric DNA molecule, comprising a polynucleotide sequence encoding a peptide represented by formula (I):
Dependencies are inferred from the claim text.