Sindbis virus expression system for recombinant antibody production
AssigneeThe Trustees of Columbia University in the City of New York
InventorBeth Cindy Levine
Life of this patent
Estimating the term…
Abstract
This invention provides a method of expressing a multimeric polypeptide. This invention also provides a method of expressing biologically active recombinant antibodies. This invention also provides an expression cell line capable of producing multimeric polypeptides or biologically active recombinant antibodies. This invention also provides methods of producing biologically active recombinant antibodies or multimeric polypeptides. This invention also provides a transgenic animal comprising the expression cell line capable of producing biologically active recombinant antibodies or multimeric polypeptides. Also, this invention provides a method for preventing infectious diseases associated with arthropods.
Claims12
A method of producing a chimeric Sindbis virus expressing immunoglobulin heavy chain protein which comprises:
- (a) obtaining the nucleic acid which encodes immunoglobulin heavy chain protein;
- (b) operatively linking the obtained nucleic acid to a Sindbis virus cDNA clone which contains all the elements, including structural genes, required for viral replication and transcription so as to form a chimeric Sindbis virus cDNA clone;
- (c) transcribing the chimeric Sindbis virus cDNA clone of step (b);
- (d) isolating the transcript of step (c);
- (e) transfecting the isolated transcripts of step (d) into a eukaryotic cell in vitro;
- and
- (f) culturing the cell of step (e) so as to produce a chimeric Sindbis virus expressing immunoglobulin heavy chain protein.
A method of producing a chimeric Sindbis virus comprising a nucleic acid molecule expressing immunoglobulin light chain protein which comprises:
- (a) obtaining the nucleic acid which encodes immunoglobulin light chain protein;
- (b) operatively linking the obtained nucleic acid to a Sindbis virus cDNA clone which contains all the elements, including structural genes, required for viral replication and transcription so as to form a chimeric Sindbis virus cDNA clone;
- (c) transcribing the chimeric Sindbis virus cDNA clone of step (b);
- (d) isolating the transcript of step (c);
- (e) transfecting the isolated transcripts of step (d) into a eukaryotic cell in vitro;
- and
- (f) culturing the cell of step (e) so as to produce a chimeric Sindbis virus expressing immunoglobulin light chain protein.
A method of expressing a biologically active recombinant antibody which comprises:
- (a) obtaining nucleic acids which encode immunoglobulin light chain protein and heavy chain protein;
- (b) operatively linking the obtained nucleic acids to Sindbis virus cDNA clones which contain all the elements, including structural genes, required for viral replication and transcription so as to form chimeric Sindbis virus cDNA clones, wherein at least one clone comprises the nucleic acid encoding immunoglobulin heavy chain and at least another clone comprises the nucleic acid encoding immunoglobulin light chain;
- (c) transcribing the chimeric Sindbis virus cDNA clones of step (b);
- (d) isolating the transcripts from step (c);
- (e) transfecting the isolated transcripts of step (d) into a eukaryotic cell in vitro;
- (f) culturing the transfected cell of step (e) so as to produce chimeric Sindbis viruses, wherein at least one virus comprises the nucleic acid encoding immunoglobulin heavy chain protein and at least another virus comprises the nucleic acid encoding immunoglobulin light chain protein;
- (g) isolating the viral vectors of step (f);
- (h) coinfecting a host cell in vitro, with the isolated viral vectors of step (g) under conditions so that the cell expresses biologically active recombinant antibodies.
Dependencies are inferred from the claim text.