Nucleic acid encoding a novel P-selectin ligand protein
AssigneeGenetics Institute, Inc.
InventorsGlenn R. Larsen, Dianne S. Sako, Xiao-Jia Chang, Geertruida M. Veldman, Dale Cumming, Ravindra Kumar, Gray Shaw
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Abstract
A novel P-selectin ligand glycoprotein is disclosed, comprising the amino acid sequence set forth in SEQ ID NO:2 or by the amino acid sequence set forth in SEQ ID NO:4. DNA sequences encoding the P-selectin ligand protein are also disclosed, along with vectors, host cells, and methods of making the P-selectin ligand protein. Pharmaceutical compositions containing the P-selectin ligand protein and methods of treating inflammatory disease states characterized by P-selectin- and E-selectin-mediated intercellular adhesion are also disclosed.
Claims27
An isolated DNA selected from the group consisting of:
- (a) a DNA encoding a P-selectin ligand protein, said protein comprising an amino acid sequence selected from the group consisting of the amino acid sequence set forth in SEQ ID NO:2 from amino acid 1 to amino acid 402, the amino acid sequence set forth in SEQ ID NO:2 from amino acid 1 to amino acid 310, the amino acid sequence set forth in SEQ ID NO:2 from amino acid 42 to amino acid 402, the amino acid sequence set forth in SEQ ID NO:2 from amino acid 42 to amino acid 310, and the amino acid sequence set forth in SEQ ID NO:4;
- and
- (b) a DNA capable of hybridizing under stringent conditions to a DNA specified in (a) and encoding a naturally occuring P-selectin ligand protein.
An isolated DNA encoding a peptide comprising amino acids 42-60 of SEQ ID NO:2.
The DNA of claim 30 wherein said peptide comprises the amino acid sequence set forth in SEQ ID NO:2 from amino acid 42 to amino acid 88.
The DNA of claim 30 wherein said peptide comprises the amino acid sequence set forth in SEQ ID NO:2 from amino acid 42 to amino acid 118.
The DNA of claim 30 wherein said peptide comprises the amino acid sequence set forth in SEQ ID NO:2 from amino acid 42 to amino acid 189.
Dependencies are inferred from the claim text.