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US 5,843,703 AGranted
Enhanced production of toxic polypeptides in prokaryotes
AssigneeCalifornia Institute of Technology
InventorsJudith L. Campbell, William C. Brown
Life of this patent
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Abstract
Compositions for enhancing the production of toxic polypeptides in prokaryotic hosts comprise a coding nucleic acid and a bacteriophage nucleic acid. The coding nucleic acid encodes a promoter operably linked to a gene encoding a selected toxic protein. The bacteriophage nucleic acid contains at least one gene from the early region of a T7-like bacteriophage other than an RNA polymerase. When both the coding nucleic acid and the bacteriophage nucleic acid are used to transform or transfect a prokaryotic host, the production of the toxic polypeptide is greater than the level of production of the same toxic polypeptide when the same host is transformed only with the coding nucleic acid.
Claims37
A prokaryotic host which expresses a toxic polypeptide, said host comprising:
- (a) a coding nucleic acid comprising a promoter operably linked to a nucleic acid coding for said toxic polypeptide;
- wherein said promoter is recognized by a RNA polymerase non endogenous to said prokaryotic host;
- and
- (b) a bacteriophage nucleic acid comprising at least one functional gene of an early region of a T7-like bacteriophage nucleic acid other than a gene encoding RNA polymerase, wherein said T7-like bacteriophage is selected from the group consisting of T1, T3, T7, .PHI.I, .PHI.II, BA14, A16, .PHI.1.2, K11, SP6, gh-1 and .PHI.Cd1, and wherein said bacteriophage nucleic acid encodes a bacteriophage which is incapable of completing its life cycle;
- and
- c) a nucleic acid encoding said non-endogenous RNA polymerase;
- wherein the level of production of said toxic polypeptide after induction in said prokaryotic host containing said nucleic acids is at least 50% greater than the level of production of said toxic polypeptide after induction in the same prokaryote containing only said coding nucleic acid.
4 more elements
A prokaryotic host which expresses a toxic polypeptide comprising:
- (a) a coding nucleic acid comprising an inducible promoter operably linked to a nucleic acid coding for said toxic polypeptide, and
- (b) a bacteriophage nucleic acid comprising at least one functional gene of an early region of a T7-like bacteriophage nucleic acid other than a gene encoding RNA polymerase, wherein said T7-like bacteriophage is selected from the group consisting of T3 and T7 and wherein said bacteriophage nucleic acid encodes a bacteriophage which is incapable of completing its life cycle,
- wherein the level of production of said toxic polypeptide after induction in said prokaryotic host containing coding and said bacteriophage nucleic acid is at least 50% greater than the level of production of said toxic polypeptide after induction in the same prokaryote containing only said coding nucleic acid.
A prokaryotic host which expresses a toxic polypeptide comprising:
- (a) a coding nucleic acid coding for said toxic polypeptide;
- and
- (b) a bacteriophage nucleic acid consisting essentially of the early region of a T7 bacteriophage,
- wherein the level of production of said toxic polypeptide after induction in said prokaryotic host containing coding and said bacteriophage nucleic acid is at least 50% greater than the level of production of said toxic polypeptide after induction in the same prokaryote containing only said coding nucleic acid.
1 more element
A prokaryotic host which expresses a toxic polypeptide comprising:
- (a) a coding nucleic acid coding for said toxic polypeptide;
- and
- (b) a bacteriophage nucleic acid comprising all early region genes of a T3 or T7 bacteriophage except for gene 1 encoding a RNA polymerase for which inclusion is optional, wherein said bacteriophage nucleic acid is mutated at least in one gene selected from the group consisting of genes 3, 5 and 6 such to be incapable of completing its life cycle,
- wherein the level of production of said toxic polypeptide after induction in said prokaryotic host containing coding and said bacteriophage nucleic acid is at least 50% greater than the level of production of said toxic polypeptide after induction in the same prokaryote containing only said coding nucleic acid.
1 more element
A prokaryotic host which expresses a toxic polypeptide comprising:
- (a) a coding nucleic acid comprising an inducible promoter operably linked to a nucleic acid coding for said toxic polypeptide;
- and
- (b) a bacteriophage nucleic acid comprising at least one functional gene of an early region of a T7 bacteriophage nucleic acid other than a gene encoding RNA polymerase, wherein said bacteriophage nucleic acid encodes a bacteriophage which is incapable of completing its life cycle,
- wherein the level of production of said toxic polypeptide after induction in said prokaryotic host containing coding and said bacteriophage nucleic acid is at least 50% greater than the level of production of said toxic polypeptide after induction in the same prokaryote containing only said coding nucleic acid.
1 more element
A method for producing a polypeptide in a prokaryotic host, said method comprising
- (a) forming a prokaryotic host comprising
- (i) a coding nucleic acid comprising an inducible promoter operably linked to a nucleic acid coding for a toxic polypeptide;
- (ii) a bacteriophage nucleic acid comprising at least one functional gene of an early region of a T7-like bacteriophage nucleic acid other than a gene encoding RNA polymerase, wherein said T7-like bacteriophage is selected from the group consisting of T1, T3, T7, .PHI.I, .PHI.II, BA14, A16, .PHI.1.2, K11, SP6, gh-1 and .PHI.Cd1, and wherein said bacteriophage, nucleic acid encodes a bacteriophage which is incapable of completing its life cycle, and
- (b) subjecting said host to conditions which permit the expression of said coding nucleic acid,
- wherein the level of production of said toxic polypeptide after induction in said prokaryotic host containing said coding and said bacteriophage nucleic acid is at least 50% greater than the level of production of said toxic polypeptide after induction in the same prokaryote containing only said coding nucleic acid.
2 more elements
A method for producing a polypeptide in a prokaryotic host, said method comprising
- (a) forming a prokaryotic host comprising a coding nucleic acid comprising a promoter operably linked to a nucleic acid coding for a toxic polypeptide, wherein said promoter is recognized by a RNA polymerase non-endogenous to said prokaryotic host;
- and
- (b) infecting said prokaryotic host with
- i) a bacteriophage nucleic acid comprising at least one functional gene of an early region of a T7-like bacteriophage nucleic;
- acid other than a gene encoding RNA polymerase, wherein said T7-like bacteriophage is selected from the group consisting of T3 and T7, and wherein said bacteriophage nucleic acid encodes a bacteriophage which is incapable of completing its life cycle;
- and
- ii) a nucleic acid encoding said RNA polymerase non-endogenous to said prokaryotic host;
- wherein said infection permits the expression of said coding nucleic acid,
- wherein the level of production of said toxic polypeptide after induction in said prokaryotic host containing coding and said bacteriophage nucleic acid is at least 50% greater than the level of production of said toxic polypeptide after induction in the same prokaryote containing only said coding nucleic acid.
6 more elements
A method for producing a polypeptide in a prokaryotic host, said method comprising
- (a) forming a prokaryotic host comprising
- (i) a coding nucleic acid comprising an inducible promoter operably linked to a nucleic acid coding for a toxic polypeptide;
- (ii) a bacteriophage nucleic acid comprising at least one functional gene of an early region of a T7 bacteriophage nucleic acid other than a gene encoding RNA polymerase, wherein said bacteriophage nucleic acid encodes a bacteriophage which is incapable of completing its life cycle, and
- (b) subjecting said host to conditions which permit the expression of said coding nucleic acid,
- wherein the level of production of said toxic polypeptide after induction said prokaryotic host containing said coding and said bacteriophage nucleic acid is at least 50% greater than the level of production of said toxic polypeptide after induction in the same prokaryote containing only said coding nucleic acid.
2 more elements
Dependencies are inferred from the claim text.