Gene expression system
AssigneeNovo Nordisk A/S
InventorsDavid John McConnell, Kevin Martin Devine, Charles O'Kane
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Abstract
The present invention relates to an expression system which comprises a phage-like bacteriocin("phibacin") or a mutant thereof, or a gene or mutant of a phibacin having a function in gene expression, such as a repressor gene, which is used to transform bacterial host for the producion of proteins, in particular in gram positive bacteria.
Claims32
An expression system for the expression of a gene product of interest comprising a phibacin or a mutant thereof, or at least one induction controlled functional operon or mutant thereof from a phibacin,the mutants having retained the induction controlled operon function, wherein said phibacin is selected from the group consisting of PBSW, PBSX, PBSY and PBSZ of Bacillus subtlis and wherein the expression of a DNA sequence coding for said product of interest is under control of said phibacin or functional operon or mutant thereof.
A DNA sequence isolated from a phibacin selected from the group consisting of PBSW, PBSX, PBSY and PBSZ of Bacillus subtilis, which encodes a repressor, or a mutant thereof having retained the ability to encode a protein having repressor function.
A DNA sequence isolated from a phibacin selected from the group consisting of PBSW, PBSX, PBSY, and PBSZ of Bacillus subtilis, which encodes a positive control factor.
A DNA sequence encoding a phibacin, wherein said phibacin is selected from the group consisting of PBSW, PBSX, PBSY and PBSz of Bacillus subtilis.
Dependencies are inferred from the claim text.