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AssigneeUniversity of Medicine and Dentistry of New Jersey
InventorsSidney Pestka, Serguei V. Kotenko
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The present invention relates to the identification of intracellular signal transduction function for a putative cytokine receptor subunit. In particular, the invention relates to the identification of the signal transduction protein for the interleukin (IL)-10 receptor. Accordingly, the present invention relates to preparing recombinant cells that express the IL-10 receptor and the newly identified IL-10 signal transduction protein, e.g., for use in screening libraries of compounds for IL-10 agonists and antagonists; to restoring IL-10 function to cells in vivo, e.g., via gene therapy; and in addition to chimeric proteins comprising this signal transduction protein to agonize IL-10 activity. In specific examples, cells transfected with both the first chain of the IL-10R and the presently identified second chain, termed herein CRFB4, were able to transduce a signal in response to contact with IL-10.
A recombinant DNA molecule comprising a sequence encoding the IL-10 receptor operably associated with an expression control sequence and a sequence encoding CRFB4 operably associated with an expression control sequence.
A transfected mammalian cell that co-expresses an IL-10 receptor and a recombinant CRFB4 protein, which cell comprises an expression vector comprising a sequence encoding CRFB4 operably associated with an expression control sequence.
Dependencies are inferred from the claim text.