Immunoassay and kit for in vitro detection of soluble DesAABB fibrin polymers
AssigneeAmerican Biogenetic Sciences, Inc.
InventorPaul E. Gargan
Life of this patent
Estimating the term…
Abstract
An in vitro immunoassay to detect and quantitate soluble crosslinked and non-crosslinked DesAABB fibrin polymers in a sample from a subject. The assay can be used to support a diagnosis of, to evaluate, and to monitor, in a mammalian subject, a thrombotic event, including, but not limited to, myocardial infarction, pulmonary embolism, stroke and deep vein thrombosis.
Claims22
A method for the in vitro detection of the presence or amount of soluble crosslinked DesAABB fibrin polymers and soluble non-crosslinked DesAABB fibrin polymers in a mammalian sample, which comprises contacting the sample with monoclonal antibody MH1 produced by hybridoma ATCC HB 9739, or an antibody that binds to the same epitope as monoclonal antibody MH1, or an immunoreactive fragment thereof to form an immunocomplex therewith and detecting the presence or amount of said immunocomplex, thereby detecting the presence or amount of said soluble crosslinked DesAABB fibrin polymers and said soluble non-crosslinked DesAABB fibrin polymers in said sample.
A method for evaluating predisposition for thrombus formation, for supporting a diagnosis of an occurrence of a thrombus, or for monitoring the formation of a thrombus in a mammal, comprising detecting the amount of soluble crosslinked DesAABB fibrin polymers and soluble non-crosslinked DesAABB fibrin polymers in a sample from said mammal, comprising contacting said sample with monoclonal antibody MH1 produced by hybridoma ATCC HB 9739, or an antibody that binds to the same epitope as monoclonal antibody MH1, or an immunoreactive fragment thereof, to form an immunocomplex therewith, detecting the amount of said immunocomplex, and comparing said amount to a control sample, to evaluate the predisposition for a thrombus formation, or the occurrence of a thrombus, or to monitor the formation of a thrombus in the mammal.
A kit for detecting in vitro the presence or amount of soluble crosslinked DesAABB fibrin polymers and soluble non-crosslinked DesAABB fibrin polymers in a sample from a mammal, which comprises:
- (a) measured amounts of standards containing said soluble crosslinked DesAABB fibrin polymers and said soluble non-crosslinked DesAABB fibrin polymers;
- (b) means for forming an immunocomplex with said soluble crosslinked DesAABB fibrin polymers and said soluble non-crosslinked DesAABB fibrin polymers, said means comprising monoclonal antibody MH1 produced by hybridoma ATCC HB 9739, or an antibody that binds to the same epitope as monoclonal antibody MH1, or an immunoreactive fragment thereof;
- and
- (c) means for detecting the amount of said immunocomplex.
Dependencies are inferred from the claim text.