Method of assaying asialoglycoprotein receptor and assay reagent therefor
AssigneeDaiichi Pure Chemicals Co., Ltd.
InventorsYoshiro Niitsu, Yutaka Kohgo, Junji Kato, Hirokazu Yago, Hisashi Hanada, Koji Ushizawa
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Abstract
The present invention is directed to a method of immunoassay of asialoglycoprotein receptors (AGPR) by bringing a specimen into contact with a monoclonal antibody that recognizes AGPR, wherein the pH of a diluted specimen solution is adjusted from 5 to 7 or phenol is added to a solution of an enzyme-labeled antibody. This method is excellent in the sensitivity and accuracy of AGPR assay and can be applied also to the screening of hepatopathy remedies.
Claims15
A method for detecting the asialoglycoprotein receptor in a sample, comprising:
- (a) contacting the sample, at a pH from 5 to 7, with an unlabeled antibody which is capable of binding the asialoglycoprotein receptor;
- and
- (b) contacting the sample from step (a) with a composition comprising an antibody which is capable of binding the asialoglycoprotein receptor and is labeled with an enzyme.
A method for detecting the asialoglycoprotein receptor in a sample, comprising:
- (a) contacting the sample with an unlabeled antibody which is capable of binding the asialoglycoprotein receptor;
- and
- (b) contacting the sample from step (a) with a composition comprising phenol and an antibody which is capable of binding the asialoglycoprotein receptor and is labeled with an enzyme, wherein the phenol accelerates the binding of the antibody which is capable of binding the asialoglycoprotein receptor and the asialoglycoprotein receptor.
A method for detecting the asialoglycoprotein receptor in a sample, comprising:
- (a) contacting the sample, at a pH from 5 to 7, with an unlabeled antibody which is capable of binding the asialoglycoprotein receptor;
- and
- (b) contacting the sample from step (a) with a composition comprising phenol and an antibody which is capable of binding the asialoglycoprotein receptor and is labeled with an enzyme.
Dependencies are inferred from the claim text.