Anti-human tyrosinase monoclonal antibody
AssigneePola Chemical Industries Inc.
InventorsHiroyuki Takimoto, Satoshi Suzuki, Koushi Shibata, Shigeki Masui
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Abstract
A mammal is immunized by using an antigen of a peptide having an amino acid sequence existing in a region having low homology to amino acid sequences of tyrosinase-related proteins but included in an amino acid sequence of human tyrosinase, and then the spleen cells of the mammal is fused with cultured cells to prepare a hybridoma producing monoclonal antibody which binds to human tyrosinase and mouse tyrosinase but does not bind to the tyrosinase-related proteins.
Claims8
A monoclonal antibody having the following properties:
- (a) it specifically binds to human tyrosinase and mouse tyrosinase, wherein said monoclonal antibody specifically binds to a peptide having a sequence within the sequence of SEQ ID NO:1 and including the amino acid sequence Asp Tyr His;
- (b) it does not specifically bind to tyrosinase-related proteins;
- and
- (c) it recognizes an epitope of Glu Asp Tyr His (SEQ ID NO:6);
- said monoclonal antibody being obtained by a method comprising the steps of:
- (i) immunizing a mammal by using a conjugate of a carrier and a peptide having the amino acid sequence listed as SEQ ID NO:1, followed by excision of spleen cells of the mammal to be fused with cultured cells, thereby preparing hybridomas;
- (ii) selecting a hybridoma among said hybridomas prepared in step (i) which produces a monoclonal antibody which specifically binds to human tyrosinase and mouse tyrosinase but does not specifically bind to tyrosinase-related proteins;
- and
- (iii) collecting an antibody protein from a supernatant of a culture obtained by culturing said hybridoma selected in step (ii) in an appropriate medium or from an ascitic fluid obtained by culturing said hybridoma in the abdominal cavity of a mouse.
Monoclonal antibody produced by the hybridoma deposited with the National Institute of Bioscience and Human Technology of the Agency of Industrial Science and Technology (Japan) under Accession No. FERM BP-4801 .
Dependencies are inferred from the claim text.