Method of screening for endometriosis
AssigneeThe Curators of the University of Missouri
InventorKathy Lynn Sharpe-Timms
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Abstract
A glycoprotein having a molecular weight of 28,000 to 32,000 and an isoelectric point of 7.0 to 9.0, both as determined by two-dimensional SDS polyacrylamide gel electrophoresis, is synthesized and secreted specifically by stromal cells of endometriotic origin. Amino acid residues in the region of the N-terminus of the glycoprotein share amino acid sequence identity with a region of tissue inhibitor of metalloproteinases-1 (TIMP-1). A method of screening for endometriosis is disclosed by detection of lower levels of the protein or TIMP-1 in peritoneal fluid or serum samples of women.
Claims2
A method of screening for endometriosis in a woman patient of reproductive age by:
- (a) obtaining a body fluid sample from the patient, the body fluid sample selected from the group consisting of peritoneal fluid and serum;
- and
- (b) detecting the presence of a protein at a level below that of normal controls in the sample wherein endometriosis is indicated in the patient, the protein characterized by
- (i) having a molecular weight of 28,000 to 32,000 as determined by two-dimensional SDS-PAGE polyacrylamide gel electrophoresis;
- (ii) having an isoelectric point of 7.0 to 9.0;
- (iii) being synthesized and secreted specifically by stromal cells of endometriotic origin;
- and
- (iv) having an N-terminal amino acid sequence as set forth in SEQ ID No:2.
A method of screening for endometriosis in a woman patient of reproductive age by:
- (a) obtaining a body fluid sample from the patient, the body fluid sample selected from the group consisting of peritoneal fluid and serum;
- and
- (b) detecting the presence of tissue inhibitor of metalloproteinases-1 (TIMP-1) in the fluid sample at a level below that of normal controls wherein endometriosis is indicated in the patient.
Dependencies are inferred from the claim text.