Methods for measuring trinucleotide repeat expansion in Saccharomyces cerevisiae
AssigneePrinceton University
InventorsVirginia Zakian, Catherine Freudenreich
Life of this patent
Estimating the term…
Abstract
Assays for measuring fragility or breakage of chromosomes at trinucleotide repeats in Saccharomyces cerevisiae are provided. These assays can also be used to measure trinucleotide repeat expansion and to screen drugs, proteins and cellular conditions which increase or decrease this expansion.
Claims4
An assay for measuring the rate of breakage or fragility of a selected trinucleotide repeat comprising inserting a selected trinucleotide repeat between two homologous sequences on a chromosome in a yeast cell culture and measuring a rate of recombination of the homologous sequences.
An assay for measuring the rate of breakage or fragility of a selected trinucleotide repeat comprising inserting a selected trinucleotide repeat between a URA3 gene and a C4A4 backup telomere sequence of a yeast artificial chromosome in a yeast cell culture and measuring a rate of loss of the URA3 gene.
Dependencies are inferred from the claim text.