Back to results
US 5,843,670 AGranted
Carbodiimide group-containing digoxigenin derivatives
AssigneeNisshinbo Industries, Inc.
InventorsOsamu Suzuki, Gen Masuda, Namiko Shiohata, Kazuko Matsumoto
Life of this patent
Estimating the term…
Abstract
As a novel digoxigenin derivative which makes the labeling procedure simple, can be used to label a naturally-occurring nucleic acid, and enables highly sensitive assay, carbodiimide group-containing digoxigenin derivative represented by the following formula is used as the label in a nucleic acid detection method or immunoassay. ##STR1##
Claims8
A carbodiimide group-containing digoxigenin derivative represented by the formula (I):
- B--Y.sup.2 --N=C=N--Y.sup.1 --W--Z (I)
- wherein Z represents a digoxigenin-containing moiety represented by the formula (Z):
- ##STR17## wherein A represents --O--, --COO--, or --NHCOO--;
- L represents a straight-chain or branched alkylene group which may have a moiety in the main chain selected from the group consisting of --NHCO--, --CONH--, --O--, --S--, --NR--,
- wherein R is an alkyl group, --COO--, and --OCO--;
- W represents a quaternary ammonium group;
- Y.sup.1 and Y.sup.2 each represents a direct bond or a straight-chain or branched alkylene group having from 1 to 20 carbon atoms in its main chain and optionally having in the main chain a moiety selected from the group consisting of --NHCO--, --CONH--, --O--, --S--, --NR--, wherein R is an alkyl group, --COO--, and --OCO--;
- and
- B represents a hydrogen atom or a monovalent organic group which may be the same as or different from that represented by --W--Z in the formula (I).
6 more elements
Dependencies are inferred from the claim text.