Loading patent…
AssigneeLumigen, Inc.
InventorsHashem Akhavan-Tafti, Katsuaki Sugioka, Yumiko Sugioka, Lekkala V. Reddy
Estimating the term…
Methods of detecting analytes or target species using two enzyme-labeled specific binding partners where the two enzymes function in concert to produce a detectable chemiluminescent signal are disclosed. The methods use a specific binding partner labeled with a hydrolytic enzyme to produce a phenolic enhancer in close proximity to a peroxidase-labeled second specific binding partner. The method is useful to detect and quantitate with improved specificity various biological molecules including antigens and antibodies by the technique of immunoassay, proteins by Western blotting, DNA by Southern blotting, RNA by Northern blotting. The method may also be used to detect DNA mutations and juxtaposed gene segments in chromosomal translocations and particularly to unambiguously identify heterozygous genotypes in a single test.
A method for simultaneously detecting a first and second target species in a sample suspected of containing both the first and second target species by a single chemiluminescent reaction comprising:
Dependencies are inferred from the claim text.