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US 5,843,663 AGranted
Methods of capturing nucleic acid analogs and nucleic acids on a solid support
AssigneeBoehringer Mannheim GmbH
InventorsChristopher John Stanley, Henrik Orum, Mikkel Jorgensen
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Abstract
A nucleic acid analog comprising a polymeric strand which includes a sequence of ligands bounds to a backbone made up of linked backbone moieties, which analog is capable of hybridization to a nucleic acid of complementary sequence, further comprising a chelating moiety capable of binding at least one metal ion by chelation.
Claims39
A method of capturing a nucleic acid analog on a solid support, the method comprising:
- (a) contacting a nucleic acid analog, at least one chelatable metal ion and a solid support comprising a solid support moiety capable of binding the at least one chelatable metal ion, the nucleic acid analog comprising
- (1) a polymeric strand portion comprising (i) a backbone comprising a plurality of linked backbone moieties and (ii) a plurality of ligands, each of the plurality of ligands being directly or indirectly bound to at least one of the plurality of linked backbone moieties;
- and
- (2) a chelating moiety, attached to the polymeric strand portion, capable of binding the at least one chelatable metal ion,
- wherein the nucleic acid analog is capable of hybridizing to a nucleic acid having a complementary sequence therewith, and
- (b) capturing the nucleic acid analog on the solid support by binding (1) the chelating moiety and the at least one chelatable metal ion and (2) the at least one chelatable metal ion and the solid support moiety.
3 more elements
A method of capturing a nucleic acid on a solid support, comprising:
- (a) hybridizing the nucleic acid with a nucleic acid analog to form a hybrid, the nucleic acid analog comprising
- (1) a polymeric strand portion comprising (i) a backbone comprising a plurality of linked backbone moieties and (ii) a plurality of ligands, each of the plurality of ligands being directly or indirectly bound to at least one of the plurality of linked backbone moieties;
- and
- (2) a chelating moiety, attached to the polymeric strand portion, capable of binding at least one chelatable metal ion;
- (b) thereafter, binding the at least one chelatable metal ion and the chelating moiety to form a chelatable hybrid complex;
- and
- (c) capturing the chelatable hybrid complex on a solid support, comprising a solid support moiety capable of binding the at least one chelatable metal ion, by binding the at least one chelatable metal ion and the solid support moiety.
4 more elements
A method of capturing a nucleic acid on a solid support, comprising:
- (a) providing a nucleic acid analog comprising
- (1) a polymeric strand portion comprising (i) a backbone comprising a plurality of linked backbone moieties and (ii) a plurality of ligands, each of the plurality of ligands being directly or indirectly bound to at least one of the plurality of linked backbone moieties;
- and
- (2) a chelating moiety, attached to the polymeric strand portion, capable of binding at least one chelatable metal ion;
- (b) thereafter, binding the at least one chelatable metal ion and the chelating moiety to form a chelatable nucleic acid analog complex;
- (c) hybridizing the nucleic acid with the chelatable nucleic acid analog complex to form a hybrid;
- and
- (d) capturing the hybrid on a solid support, comprising a solid support moiety capable of binding the at least one chelatable metal ion, by binding the at least one chelatable metal ion and the solid support moiety.
5 more elements
A method of capturing a nucleic acid on a solid support, comprising:
- (a) hybridizing the nucleic acid with a nucleic acid analog to form a hybrid, the nucleic acid analog comprising
- (1) a polymeric strand portion comprising (i) a backbone comprising a plurality of linked backbone moieties and (ii) a plurality of ligands, each of the plurality of ligands being directly or indirectly bound to at least one of the plurality of linked backbone moieties;
- and
- (2) a chelating moiety, attached to the polymeric strand portion, capable of binding at least one chelatable metal ion;
- and
- (b) thereafter, capturing the hybrid on a solid support, comprising the at least one chelatable metal ion, by binding the at least one chelatable metal ion and the chelating moiety.
3 more elements
A method of capturing a nucleic acid on a solid support, comprising:
- (a) providing a solid support comprising a nucleic acid analog bound to the solid support by at least one chelatable metal ion, the nucleic acid analog comprising
- (1) a polymeric strand portion comprising (i) a backbone comprising a plurality of linked backbone moieties and (ii) a plurality of ligands, each of the plurality of ligands being directly or indirectly bound to at least one of the plurality of linked backbone moieties;
- and
- (2) a chelating moiety, attached to the polymeric strand portion, bound to the at least one chelatable metal ion;
- and
- (b) thereafter, capturing the nucleic acid on the solid support by hybridizing the nucleic acid with the nucleic acid analog.
3 more elements
Dependencies are inferred from the claim text.