Method, device, and kit for total nucleic acid assay
AssigneeGeno Technology, Inc.
InventorsAlan Dean, Aftab Alam
Life of this patent
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Abstract
A method, device and a kit for determining total nucleic acid concentration in a nucleic acid solution, which comprises applying the nucleic acid solution to a nucleic acid-support capable of retaining the total nucleic acid in the form of a nucleic acid spot having a size that is proportional to the nucleic acid concentration; measuring the size of the nucleic acid spot produced on the nucleic-support; and comparing the size of the nucleic acid spot with a standard.
Claims19
A method for determining the concentration of nucleic acid in a nucleic acid-containing solution, comprising:
- applying the nucleic acid-containing solution to a nucleic acid-support, wherein nucleic acid of the nucleic acid-containing solution contacts the nucleic acid-support in the presence of an aqueous medium having a pH effective to form a substantially uniform nucleic acid spot having a size that is proportional to the concentration of nucleic acid in the nucleic acid-containing solution;
- detecting the nucleic acid spot with a nucleic acid dye, nucleic acid stain, chemiluminescent reaction or immunological reaction;
- and
- measuring the size of the nucleic acid spot;
- and
- comparing the measured size of the nucleic acid spot with a standard, thereby determining the concentration of nucleic acid in the nucleic acid-containing solution.
A kit for determining the concentration of nucleic acid in a nucleic acid-containing solution, comprising:
- a nucleic acid-support for binding and retaining nucleic acid from a nucleic acid-containing solution applied to the nucleic acid support, wherein the nucleic acid-support is capable of retaining the nucleic acid in the form of a substantially circular nucleic acid spot having a diameter that is proportional to the concentration of nucleic acid in the solution;
- a means for measuring nucleic acid spot diameter, wherein said means is a gauge comprising a display which shows a substantially linear relationship between the diameter of the nucleic acid spot and the amount of nucleic acid in the spot and a calliper;
- and
- a nucleic acid detecting means selected from the group consisting of:
- a nucleic acid dye, a nucleic acid stain, reagents for performing a chemiluminescent reaction of reagents for performing an immunological reaction.
Dependencies are inferred from the claim text.