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US 5,843,647 AGranted
Simple tandem repeats
AssigneeUniversity of Leicester
InventorsAlec John Jeffreys, John Armour
Life of this patent
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AssigneeUniversity of Leicester
InventorsAlec John Jeffreys, John Armour
Estimating the term…
The present invention concerns a method for the identification from DNA of a fragment comprising a simple tandem repeat locus comprising the steps of: i) contacting a DNA library with at least one hybridisation probe so as to identify a population of DNA fragments enriched for simple tandem repeats; ii) isolating and cloning said population; and iii) screening of the resulting DNA library so as to identify an individual fragment comprising a simple tandem repeat locus. Also provided are simple tandem repeats isolated by the method of the present invention, characterised in that they may be amplified at least in part by PCR using a specified pair of primers, together with amplification primers and probes specific to the simple tandem repeats so isolated. The present invention also provides methods of genetic characterisation using the aforementioned simple tandem repeats, primers and probes.
A simple tandem repeat for use in a method of diagnosis of the human or animal body characterised in that it may be amplified at least in part by PCR using any one of primer pairs 3 (SEQ ID NO:56 and 57), 5 (SEQ ID NO:60 and 61), 8 (SEQ ID NO:66 and 67), 13 (SEQ ID NO:76 and 77), 16 (SEQ ID NO:82 and 83), 17 (SEQ ID NO:84 and 85), 21 (SEQ ID NO:92 and 93), 28 (SEQ ID NO:106 and 107), 29 (SEQ ID NO:108 and 109), 30 (SEQ ID NO:110 and 111), 31 (SEQ ID NO:112 and 113), 32 (SEQ ID NO:114 and 115), 33 (SEQ ID NO:116 and 117), 34 (SEO ID NQ:118 and 119), 35 (SEQ ID NO:120 and 121), 36 (SEQ ID NO:122 and 123), and 37 (SEQ ID NO:124 and 125).
Dependencies are inferred from the claim text.