DNA molecules encoding murine son of sevenless (mSOS) gene and mSOS polypeptides
AssigneeHoward Florey Institute of Experimental Physiology and Medicine
InventorDavid Douglas Lawrence Bowtell
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Abstract
Isolated DNA molecules comprising a nucleotide sequence encoding murine son of sevenless gene 1 (mSOS1) polypeptide and comprising a nucleotide sequence encoding murine son of sevenless gene 2 (mSOS2) polypeptide are disclosed, as well as isolated mSOS1 polypeptide and isolated mSOS2 polypeptide, and diagnostic methods using the same.
Claims13
An isolated DNA molecule comprising a nucleotide sequence encoding murine son of sevenless gene 1 (mSOS1) polypeptide having the amino acid sequence of SEQ ID NO:2, or a fragment of at least 20 nucleotides of said DNA molecule.
An isolated DNA molecule comprising a nucleotide sequence encoding murine son of sevenless gene 2 (mSOS2) polypeptide having the amino acid sequence of SEQ ID NO:4, or a fragment of at least 20 nucleotides of said DNA molecule.
An isolated mSOS1 polypeptide having the amino acid sequence shown in SEQ ID NO:2, or a fragment of at least 20 amino acids thereof.
An isolated mSOS2 polypeptide having the amino acid sequence shown in SEQ ID NO:4, or a fragment of at least 20 amino acids thereof.
A method for detecting a mutant mSOS1 gene comprising the steps of:
- (A) obtaining DNA encoding mSOS1 protein from a test subject;
- (B) determining:
- (i) the nucleotide sequence, and optionally the encoded amino acid sequence, of the resulting DNA obtained in step (A), or
- (ii) the chromosomal location of the resulting DNA obtained in step (A), or
- (iii) the structure of the resulting DNA obtained in step (A);
- (C) comparing the resulting nucleotide sequence or chromosomal location or structure obtained in step (B) with the nucleotide sequence, chromosomal location or structure of wild-type mSOS1 gene, wherein the nucleotide sequence of wild-type mSOS1 gene is that shown in SEQ ID NO:1, or comparing the resulting encoded amino acid sequence with the amino acid sequence of wild-type mSOS1 protein, wherein the amino acid sequence of wild-type mSOS1 protein is that shown in SEQ ID NO:2,
- wherein a mutant mSOS1 gene is detected when the resulting nucleotide sequence, chromosomal location or structure obtained in step (B) differs from that of SEQ ID NO:1, or when the resulting encoded amino acid sequence obtained in step (B) differs from that of SEQ ID NO:2.
A method for detecting a mutant mSOS2 gene comprising the steps of:
- (A) obtaining DNA encoding mSOS2 protein from a test subject;
- (B) determining:
- (i) the nucleotide sequence, and optionally the encoded amino acid sequence, of the resulting DNA obtained in step (A), or
- (ii) the chromosomal location of the resulting DNA obtained in step (A), or
- (iii) the structure of the resulting DNA obtained in step (A);
- (C) comparing the resulting nucleotide sequence or chromosomal location or structure obtained in step (B) with the nucleotide sequence, chromosomal location or structure of wild-type mSOS1 gene, wherein the nucleotide sequence of wild-type mSOS2 gene is that shown in SEQ ID NO:3, or comparing the resulting encoded amino acid sequence with the amino acid sequence of wild-type mSOS2 protein, wherein the amino acid sequence of wild-type mSOS1 protein is that shown in SEQ ID NO:4,
- wherein a mutant mSOS2 gene is detected when the resulting nucleotide sequence, chromosomal location or structure obtained in step (B) differs from that of SEQ ID NO:3, or when the resulting encoded amino acid sequence obtained in step (B) differs from that of SEQ ID NO:4.
A method for detecting a mutant mSOS1 protein comprising the steps of:
- (A) obtaining mSOS1 protein from a test subject;
- (B) determining the amino acid sequence of the resulting mSOS1 protein obtained in step (A);
- (c) comparing the resulting amino acid sequence obtained in step (B) with the amino acid sequence of wild-type mSOS1 protein, wherein the amino acid sequence of wild-type mSOS1 protein is that shown in SEQ ID NO:2,
- wherein a mutant mSOS1 protein is detected when the resulting amino acid sequence obtained in step (B) differs from that of SEQ ID NO:
- 2.
A method for detecting a mutant mSOS2 protein comprising the steps of:
- (A) obtaining mSOS2 protein from a test subject;
- (B) determining the amino acid sequence of the resulting mSOS2 protein obtained in step (A);
- (C) comparing the resulting amino acid sequence obtained in step (B) with the amino acid sequence of wild-type mSOS2 protein, wherein the amino acid sequence of wild-type mSOS2 protein is that shown in SEQ ID NO:4,
- wherein a mutant mSOS2 protein is detected when the resulting amino acid sequence obtained in step (B) differs from that of SEQ ID NO:4.
Dependencies are inferred from the claim text.