Adhesin-oligosaccharide conjugate vaccine for Haemophilus influenzae
AssigneeAntexBiologics, Inc.
InventorsHoward C. Krivan, James E. Samuel
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Abstract
Disclosed herein are immunogenic polysaccharide-H. influenzae adhesin protein conjugates, a purified H. influenzae adhesin protein and related proteins and polypeptides, DNA useful for producing the proteins, synthetic polyribosylribotol phosphate (PRP) oligosaccharides and intermediates useful for their synthesis, and methods of making and using these materials. The conjugates comprise a PRP fragment, preferably a synthetic oligosaccharide, coupled to an H. influenzae adhesin protein. The invention further comprises purified H. influenzae adhesin proteins and novel PRP oligosaccharides. The invention also comprises methods of producing these materials and using them in a vaccine to protect humans and other mammals against H. influenzae infection.
Claims16
A purified H. influenzae protein comprising Sequence ID No. 2.
A purified H. influenzae protein comprising amino acids designated 26 to 463 in Sequence ID No. 2.
A recombinant protein produced by a host cell transformed with an isolated or substantially purified DNA comprising nucleotides designated 115 to 1503 in Sequence ID No. 1 encoding H. influenzae protein operably linked to appropriate regulatory control nucleic acid sequences that are capable of effecting expression of said DNA in said transformed host cell.
A recombinant protein produced by a host cell transformed with an isolated or substantially purified DNA comprising nucleotides designated 190 to 1503 in Sequence ID No. 1 encoding H. influenzae protein operably linked to appropriate regulatory control nucleic acid sequences that are capable of effecting expression of said DNA in said transformed host cell.
Dependencies are inferred from the claim text.