Blood coagulation protein antagonists and uses therefor
AssigneeCorvas International, Inc.
InventorsHoward R. Soule, Terence K. Brunck
Life of this patent
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Abstract
Coagulation protein antagonists are disclosed, which include monoclonal-type antibodies and related cell lines disclosed for the production of specific, neutralizing antibodies against factors VII and VIIa and the tissue factor/factor VIIa bimolecular complex, which antibodies are useful for the prevention or treatment of thrombotic and related diseases, for immunoaffinity isolation and purification of factors VII and VIIa and the tissue factor/factor VIIa complex, and for determination of factors VII or VIIa and the tissue factor/factors VII or VIIa complex in a biological sample.
Claims18
A method of treating a mammalian species for a thrombotic disease condition that would be inhibited by an agent that selectively interferes with the coagulation cascade, which comprises administering to a mammalian species in need of such treatment a therapeutically effective amount of an agent selected from the group consisting of:
- (i) monoclonal-antibody 12D10 produced by hybridoma cell line ATTC HB 10558:
- (ii) monoclonal antibody active at the epitope bound by monoclonal antibody 12D10 that is a tissue factor:factor VII and/or tissue factor:factor VIIa complex antagonist, (iii) an epitope binding fragment of monoclonal antibody 12D10, and (iv) a functional fragment of a monoclonal antibody active at the epitope bound by monoclonal antibody 12D10 wherein said functional fragment specifically binds with said complex but does not compromise hemostasis by inhibiting free factor VII from binding to tissue factor.
A method of inhibiting the procoagulant activity of the tissue factor:factor VIIa complex in vivo which comprises administering to a mammalian species a therapeutically effective amount of an agent selected from the group consisting of:
- (i) monoclonal antibody 12D10 produced by hybridoma cell line ATTC HB 10558, (ii) monoclonal antibody active at the epitope bound by monoclonal antibody 12D10, (iii) a epitope binding fragment of monoclonal antibody 12D10, and (iv) a functional fragment of a monoclonal antibody active at the epitope bound by monoclonal antibody 12D10 wherein said functional fragment specifically binds with said complex but does not compromise hemostasis by inhibiting free factor VII from binding to tissue factor and thereby inhibiting procoagulant activity following conversion of factor VII to factor VIIa.
Dependencies are inferred from the claim text.