Transplantation and graft-versus-host-disease
AssigneeThe General Hospital Corporation
InventorsDavid H. Sachs, J. Scott Arn, Thomas Lorf
Life of this patent
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Abstract
A method of preparing swine donor tissue which includes hematopoietic stem cells and T cells for transplantation into a recipient mammal other than a swine. The method includes the swine donor tissue with an antibody which binds the epitope recognized by the mAb 2-6-15 monoclonal antibody. The binding facilitates depletion of T cells about as efficiently or more efficiently than does the mAb 2-6-15 monoclonal antibody and results in about the same or less depletion of stem cells as does the mAb 2-6-15 monoclonal antibody.
Claims19
A method of preparing swine donor tissue which comprises hematopoietic stem cells and T cells for tranplantation into a recipient mammal other than a swine, comprising:
- contacting said swine donor tissue which comprises hematopoietic stem cells and T cells with an antibody which binds the epitope recognized by the mAb 2-6-15 monoclonal antibody and wherein said binding faciltates depletion of said T cells about as efficiently or more efficently than does the mAb 2-6-15 monoclonal antibody while resulting in about the same or less depletion of stem cells as does the the mAb 2-6-15 monoclonal antibody, thereby preparing swine donor tissue for transplantation into a recipient mammal other than a swine.
A preparation comprising hematopoietic stem cells, swine T cells, and an antibody which binds the epitope recognized by the mAb 2-6-15 monoclonal antibody and wherein said binding facilitates depletion of said T cells about as efficiently or more efficiently than does the mAb 2-6-15 monoclonal antibody while resulting in about the same or less depletion of stem as does the mAb 2-6-15 monoclonal antibody.
A preparation of T cell-depleted swine hematopoietic stem cells, wherein the preparation is made by a method comprising contacting a population of swine hematopoietic stem cells and swine T cells with an antibody which binds the epitope recognized by the mAb 2-6-15 monoclonal antibody and wherein said binding facilitates depletion of said T cells about as efficiently or more efficiently than does the mAb 2-6-15 monoclonal antibody while resulting in about the same or less depletion of stem cells as does the mAb 2-6-15 monoclonal antibody, thereby depleting the T cells.
A method of reducing graft-versus-host disease in a mammal which is the recipient of a swine graft, comprising:
- introducing "a preparation comprising swine bone marrow cells and swine T cells to" said recipient mammal;
- implanting said swine graft into said recipient mammal;
- and
- administering to said recipient mammal an antibody which binds the epitope recognized by the mAb 2-6-15 monoclonal antibody and wherein said binding facilitates depletion of said T cells about as efficiently or more efficiently than does the mAb 2-6-15 monoclonal antibody while resulting in about the same or less depletion of stem cells as does the mAb 2-6-15 monoclonal antibody,
- whereby said graft-versus-host disease is reduced in comparison to engraftment in the absence of treatment with said antibody.
A method of reducing graft-versus-host discase in a mammal which is the recipient of a swine graft, comprising:
- contacting a preparation which comprises swine bone marrow cells and swine T cells with an antibody which binds the epitope recognized by the mAb 2-6-15 monoclonal antibody and wherein said binding facilitates depletion of said T cells about as efficiently or more efficiently than does the mAb 2-6-15 monoclonal antibody while resulting in about the same or less depletion of stem cells as does the mAb 2-6-15 monoclonal antibody;
- introducing said antibody-treated preparation into said mammal;
- and
- implanting said swine graft into said recipient mammal,
- whereby said graft-versus-host disease is reduced in comparison to engraftment with swine bone marrow cells which have not been treatment said antibody.
A method of reducing graft-versus-host disease in a mammal which is the recipient of a swine graft, comprising:
- contacting a preparation which comprises swine bone marrow cells and swine T cells with an antibody which binds the epitope recognized by the mAb 2-6-15 monoclonal antibody and wherein said binding facilitates depletion of said T cells about as efficiently or more efficiently than does the mAb 2-6-15 monoclonal antibody while resulting in about the same or less depletion of stem as does the mAb 2-6-15 monoclonal antibody;
- introducing said antibody-treated preparation into said recipient mammal;
- implanting said swine graft into said recipient mammal;
- and
- administering to said recipient mammal an antibody which binds the epitope recognized by the mAb 2-6-15 monoclonal antibody and wherein said binding facilitates depletion of said T cells about efficiently or more efficently than the mAb 2-6-15 monoclonal antibody while resulting in about the same or less depletion of stem cells as the mAb 2-6-15 monoclonal antibody,
- whereby said graft-versus-host discase is reduced in comparison to engraftment in the absence of treatment with said antibody.
Dependencies are inferred from the claim text.