Detection of hypoxia
AssigneesThe Trustees of the University of Pennsylvania, Trustees of the University of Rochester
InventorsCameron J. Koch, Edith M. Lord
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Abstract
Novel nitroaromatic compounds and immunogenic conjugates comprising a novel nitroaromatic compound and a carrier protein are disclosed. The invention further presents monoclonal antibodies highly specific for the claimed nitroaromatic compounds, the compounds' protein conjugates, the compounds' reductive byproducts, and adducts formed between the compounds and mammalian hypoxic cell tissue proteins. The invention is further directed to methods for detecting tissue hypoxia using immunohistological techniques, non-invasive nuclear medicinal methods, or nuclear magnetic resonance. Diagnostic kits useful in practicing the methods of claimed invention are also provided.
Claims15
A method for preparing a monoclonal antibody comprising:
- a) introducing into a mammal a compound bound to a protein, the compound having the formula:
- ##STR3## wherein R.sub.1 is CH.sub.2 and R.sub.2 is an alkyl group having up to about 6 halogen atoms, wherein said alkyl group has the formula CHXCX.sub.2 CY.sub.3 where X is halogen or hydrogen and Y is fluorine or bromine;
- and
- b) fusing immune cells of the mammal with mammalian myeloma cells forming a hybridoma that produces antibodies specific for the compound bound to the protein.
A monoclonal antibody specific for a compound, the compound's protein conjugate, the compound's reductive byproduct, or adduct formed between the compound and tissue protein, the compound having the formula:
- ##STR4## wherein R.sub.1 is CH.sub.2 and R.sub.2 is an alkyl group having up to about 6 halogen atoms, wherein said alkyl group has the formula CHXCX.sub.2 CY.sub.3 where X is halogen or hydrogen and Y is fluorine or bromine.
Dependencies are inferred from the claim text.