Chromatography material
AssigneeGenoMed, LLC
InventorsThomas Manz, Jochen Tittgen
Life of this patent
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Abstract
A chromatography material is described for separation of nucleic acid mixtures in which a support is converted with a silanization reagent, in which the silanization reagent has a reactive group converted with silanization reagent, in which the silanization reagent has a reactive group converted with an alkyl- or dialkylamine, or contains a reactive group that can be converted with an alkyl- or dialkylamine, which is then reacted with the alkyl- or dialkylamine.
Claims10
A process for chromatographic separation of DNA from RNA of a mixture wherein both RNA and DNA are present, with chromatography material wherein the chromatography material comprises:
- a support that has been reacted with a silanization agent selected from the group consisting of
- (1) a silanization reagent having a reactive group converted with an alkyl- or dialkylamine and
- (2) a silanization reagent that contains a reactive group that can be converted with an alkyl- or dialkylamine, which support has thereafter been reacted with the alkyl- or dialkylamine, said support having a particle size of 1 to 500 .mu.m and a pore diameter between 3 to 6 nm,
- the process comprising the steps of:
- loading the mixture on the chromatography material;
- washing the loaded chromatographic material, and
- applying an eluent comprising a salt solution to the chromatographic material.
Dependencies are inferred from the claim text.