Alternative open reading frame DNA of a normal gene and a novel human cancer antigen encoded therein
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsRong-Fu Wang, Steven A. Rosenberg
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Abstract
The present invention discloses that the normal melanogenic gene, gp75 gene, encodes a gene product, a 24 amino acid peptide of ORF3, which is processed to an antigenic cancer peptide recognized by T lymphocytes. The cancer peptide of the invention derived from ORF3 is recognized by cancer antigen specific T lymphocytes as a tumor rejection antigen. The products of this gene are promising candidates for immunotherapeutic strategies for the treatment and diagnosis of patients with cancer.
Claims20
A cancer antigen peptide or antigenic epitope thereof encoded by an alternative open reading frame nucleic acid sequence of the TRP-1 gene other than the open reading frame sequence that encodes the normal TRP-1 protein wherein the nucleic acid sequence consists of ORF3 depicted in FIG. 2 as SEQ ID No:
- 5 or a portion thereof.
A cancer antigen peptide or antigenic epitope thereof comprising an amino acid sequence:
- MXaaLQRQFLRTQLWDVPSWLERSCL (SEQ. ID NO:
- 7) or antigenic portion thereof, wherein Xaa=Ser or Ala;
- or a variant of SEQ. ID NO:
- 7 having a substitution of an amino acid at no more than three amino acid positions in SEQ. ID NO:
- 7, said variant having the biological function of stimulating cancer antigen specific T lymphocytes.
A cancer antigen peptide or antigenic epitope thereof comprising the amino acid sequence:
- MSLQRQFLR (SEQ ID NO:
- 9) or antigenic portion thereof.
Dependencies are inferred from the claim text.