Aspartic proteinase deficient filamentous fungi
OwnerRandy M. Berka, Kirk J. Hayenga +2inventors
InventorsRandy M. Berka, Kirk J. Hayenga, Virgil B. Lawlis, Michael Ward
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Abstract
This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypeptides such as chymosin.
Claims10
A viable mutant Aspergillus selected from the group consisting of A. niger, A. awamori, and A. oryzae suitable for the production of heterologous polypeptides, wherein the mutant comprises a site-selected deletion of part or all of nucleic acid coding for an aspergillopepsin such that said mutant does not excrete said aspergillopepsin and does not revert to wild type, wherein said nucleic acid coding for said aspergillopepsin hybridizes to an oligonucleotide probe coding for six or more contiguous amino acids of the amino acid sequence of aspergillopepsin A from A. awamori.
Dependencies are inferred from the claim text.