Ingap protein involved in pancreatic islet neogenesis
AssigneesMoGill University, Eastern Virginia Medical School of the Medicine College of Hampton Roads
InventorsAaron I. Vinik, Gary L. Pittenger, Ronit Rafaeloff, Lawrence Rosenberg, William P. Duguid
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Abstract
Cellophane wrapping (CW) of hamster pancreas induces proliferation of duct epithelial cells followed by endocrine cell differentiation and islet neogenesis. Using the mRNA differential display technique a cDNA clone expressed in cellophane wrapped but not in control pancreata was identified. Using this cDNA as a probe, a cDNA library was screened and a gene not previously described was identified and named INGAP.
Claims19
An isolated DNA molecule encoding a mammalian islet cell neogenesis associated protein (INGAP) protein, wherein the INGAP protein has the amino acid sequence shown in SEQ ID NO:
- 2.
A nucleotide probe comprising at least 30 contiguous nucleotides of a sequence encoding a mammalian islet cell neogenesis associated protein (INGAP), wherein said protein has the sequence shown in SEQ ID NO:
- 2.
A DNA molecule comprising at least 30 contiguous nucleotides of a sequence encoding a mammalian islet cell neogenesis associated protein (INGAP), wherein said protein has the sequence shown in SEQ ID NO:
- 2.
An antisense construct of a mammalian islet cell neogenesis associated protein (INGAP) gene comprising:
- a promoter, a terminator, and a nucleotide sequence consisting of a mammalian INGAP gene, wherein the gene encodes a protein as shown in SEQ ID NO:
- 2, said nucleotide sequence being between said promoter and said terminator, said nucleotide sequence being inverted with respect to said promoter, whereby upon expression from said promoter an mRNA complementary to native mammalian INGAP mRNA is produced.
Dependencies are inferred from the claim text.