Method for inhibiting thrombosis in a patient whose blood is subjected to extracorporeal circulation
AssigneeThe Trustees of Columbia University in the City of New York
InventorsEric Rose, David Stern, Ann Marie Schmidt, Talia Spanier
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Abstract
This invention provides an assay to monitor antithrombic activity of a Factor IXa compound infused into circulation of a patient which includes: (a) obtaining Factor IXa-deficient plasma; (b) mixing the plasma from step (a) with diatomaceous and with plasma from the patient; (c) incubating the mixture with a source of lipid and calcium chloride under conditions suitable for clot formation; and (d) measuring time necessary for clot formation in the incubate, thereby monitoring the antithrombic activity of the Factor IXa compound infused into the circulation of the patient.
Claims10
An assay to determine the anticoagulant activity of a Factor IXa compound on a subject's blood which comprises:
- (a) mixing Factor IX deficient plasma, diatomaceous earth and plasma containing the Factor IXa compound derived from the subject's blood;
- (b) incubating the resulting mixture from step (a) with an effective dose of a source of phospholipid and calcium chloride under conditions such that clot formation results;
- and
- (c) measuring the time necessary for clot formation in the incubate of step (b) so as to thereby determine the anticoagulant activity of the Factor IXa compound.
A method for monitoring the anticoagulant activity of a Factor IXa compound infused into the circulation of a subject's blood during surgery which comprises measuring the anticoagulant activity of the Factor IXa compound at different times during the surgery using the assay of claim 23 and comparing the activities so measured.
Dependencies are inferred from the claim text.