Oligonucleotide N3'-P5' phosphoramidates: hybridization and nuclease resistance properties
AssigneeLynx Therapeutics, Inc.
InventorsSergei M. Gryaznov, Ronald G. Schultz, Jer-kang Chen
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Abstract
Modified oligonucleotides 3'-NHP(O)(O.sup.-)O-5' phosphoramidates were synthesized on a solid phase support. The phosphoramidate analogs were found to have significantly increased resistance toward phosphodiesterase digestion. Thermal dissociation experiments demonstrated that these compounds form more stable duplexes than phosphodiesters with complementary DNA and particularly RNA strands. Further, the phosphoramidate analogs can also form stable triplexes with double-stranded DNA target, where under similar conditions parent phosphodiester compounds failed to do so.
Claims7
An oligodeoxyribonucleotide comprising a defined sequence of nucleoside subunits joined by inter-subunit linkages and defined by the formula:
- ##STR1## wherein B is a purine or pyrimidine or an analog thereof and n is between 2 and 48.
Dependencies are inferred from the claim text.