Culture medium and methods for detecting staphylococci

A method for detecting staphylococci in a sample, comprising:

• culturing said sample in a medium containing components selective for growing staphylococci and egg-yolk as a differential detection agent under conditions to produce detectable colonies, said medium further comprising a glucopyranoside indicator substance in an amount sufficient to distinguish colonies containing staphylococci from colonies containing Bacillus colonies when said sample is cultured in said medium.

A method for detecting staphylococci in a sample, comprising:

• culturing said sample in a medium containing components selective for growing staphylococci under conditions to produce detectable colonies, said medium comprising a glucopyranoside indicator substance in an amount sufficient to distinguish colonies containing staphylococci from colonies containing Bacillus colonies when said sample is cultured in said medium;
• and
• confirming the presence or absence of staphylococci in said sample by determining whether or not colonies grown from said sample exhibit coagulase activity.

A method for detecting staphylococci in a sample, comprising:

• culturing said sample in a medium containing components selective for growing staphylococci under conditions to produce detectable colonies, said medium comprising a glucopyranoside indicator substance in an amount sufficient to distinguish colonies containing staphylococci from colonies containing Bacillus colonies when said sample is cultured in said medium;
• and
• confirming the presence or absence of staphylococci in said sample by determining whether or not colonies grown from said sample exhibit thermostable nuclease activity.