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US 5,837,473 AGranted
Methods of screening for agents affecting the deposition of .beta.-amyloid peptides on amyloid plaques in human tissue
AssigneesPresident and Fellows of Harvard College, Regents of the University of Minnesota
InventorsJohn Edward Maggio, Patrick William Mantyh
Life of this patent
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Abstract
The present invention provides a labelled .beta.-amyloid peptide or a labelled active fragment of .beta.-amyloid peptide and methods of using the labelled peptides to screen for agents which affect the deposition of the labelled peptide onto amyloid plaques in tissue samples evidencing the presence of Alzheimer's amyloidosis.
Claims7
An in vitro method of screening for an agent capable of affecting the deposition of .beta.-amyloid peptide or peptide fragment thereof on tissue, the method comprising:
- (a) combining a sample of human tissue evidencing the presence of Alzheimer's amyloidosis, with an amount of labelled .beta.-amyloid peptide or labelled active peptide fragment thereof and an agent to be screened for a time effective to allow binding of the labelled .beta.-amyloid peptide of the labelled fragment thereof to amyloid plaques in the tissue;
- (b) detecting the amount of labelled .beta.-amyloid peptide or labelled peptide fragment bound to the tissue sample;
- and
- (c) assessing the effect the agent has on .beta.-amyloid peptide or peptide fragment deposition.
1 more element
Dependencies are inferred from the claim text.