GnRH-leukotoxin chimeras
AssigneeUniversity of Saskatchewan
InventorsAndrew A. Potter, John G. Manns
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Abstract
New immunological carrier systems, DNA encoding the same, and the use of these systems, are disclosed. The carrier systems include chimeric proteins which include a leukotoxin polypeptide fused to one or more selected GnRH multimers which comprise at least one repeating GnRH decapeptide sequence, or at least one repeating unit of a sequence corresponding to at least one epitope of a selected GnRH molecule. Under the invention, the selected GnRH sequences may all be the same, or may correspond to different derivatives, analogues, variants or epitopes of GnRH so long as the GnRH sequences are capable of eliciting an immune response. The leukotoxin functions to increase the immunogenicity of the GnRH multimers fused thereto.
Claims23
A chimeric protein comprising a leukotoxin polypeptide fused to first and second multimers, wherein the C-terminus of the first multimer is fused to the N-terminus of the leukotoxin polypeptide and the N-terminus of the second multimer is fused to the C-terminus of the leukotoxin polypeptide, and further wherein each of said multimers comprises more than one selected GnRH polypeptide.
Dependencies are inferred from the claim text.