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AssigneeFordham University
InventorPramod K. Srivastava
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The present invention relates to methods and compositions for eliciting an immune response and the prevention and treatment of primary and metastatic neoplastic diseases and infectious diseases. The methods of the invention comprise administering a composition comprising an effective amount of a complex, in which the complex consists essentially of a heat shock protein (hsp) noncovalently bound to an antigenic molecule. "Antigenic molecule" as used herein refers to the peptides with which the hsps are endogenously associated in vivo as well as exogenous antigens/immunogens (i.e., with which the hsps are not complexed in vivo) or antigenic/immunogenic fragments and derivatives thereof. In a preferred embodiment, the complex is autologous to the individual. The effective amounts of the complex are in the range of 10-600 micrograms for complexes comprising hsp70, 50-1000 micrograms for hsp90, and 10-600 micrograms for gp96. The invention also provides a method for measuring tumor rejection in vivo in an individual, preferably a human, comprising measuring the generation by the individual of MHC Class I-restricted CD8+ cytotoxic T lymphocytes specific to the tumor. Methods of purifying hsp70-peptide complexes are also provided.
A method of eliciting an immune response in a human individual of greater than 20 kg body weight comprising administering to the individual a composition comprising an amount of a purified complex in the range of 10 to 600 micrograms, said complex consisting essentially of heat shock protein gp96 noncovalently bound to an antigenic molecule.
A method of treating a human individual of greater than 20 kg body weight having a type of cancer, comprising administering to the individual a composition comprising an amount of a purified complex in the range of 10 to 600 micrograms, said complex consisting essentially of heat shock protein gp96 noncovalently bound to a tumor-specific antigenic molecule specific to said type of cancer.
A method of treating a human individual of greater than 20 kg body weight having a type of cancer, comprising:
A method of inhibiting growth and progression of a type of cancer in a human individual of greater than 20 kg body weight in whom such inhibition is desired, comprising administering to the individual a composition comprising an amount of a purified complex in the range of 10 to 600 micrograms, said complex consisting essentially of heat shock protein gp96 noncovalently bound to a tumor-specific antigenic molecule specific to said type of cancer.
A kit comprising in a container a composition comprising an amount of purified complex in the range of 10 to 600 micrograms, said complex consisting essentially of heat shock protein gp96 noncovalently bound to an antigenic molecule.
A method of treating a human individual of greater than 20 kg body weight having a type of cancer, comprising administering to the individual a composition comprising an amount of a purified complex in the range of 10 to 600 micrograms, said complex consisting essentially of heat shock protein gp96 noncovalently bound to an antigenic molecule, wherein the complex is obtained from tissue of said type of cancer.
A method of treating a human individual of greater than 20 kg body weight having a type of cancer, comprising administering to the individual a composition comprising an amount of a purified complex in the range of 10 to 600 micrograms, said complex consisting essentially of heat shock protein gp96 noncovalently bound to an antigenic molecule, wherein the complex is obtained from a tumor cell line of said type of cancer.
A method of inhibiting Cancer Growth and Progression in a human individual of greater than 20 kg body weight in whom such inhibition of a type of cancer is desired, comprising administering to the individual a composition comprising an amount of a purified complex in the range of 10 to 600 micrograms, said complex consisting essentially of heat shock protein gp96 noncovalently bound to an antigenic molecule, wherein the complex is obtained from tissue of said type of cancer.
A method of inhibiting Cancer Growth and Progression in a human individual of greater than 20 kg body weight in whom such inhibition of a type of cancer is desired, comprising administering to the individual a composition comprising an amount of a purified complex in the range of 10 to 600 micrograms, said complex consisting essentially of heat shock protein gp96 noncovalently bound to an antigenic molecule, wherein the complex is obtained from a tumor cell line of said type of cancer.
Dependencies are inferred from the claim text.